| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Madison G (12)
|
VAGATR |
28.63 |
29.27 |
0.64 |
| 2 |
Agh, Lelle (12)
|
VAGATR |
30.00 |
29.40 |
-0.60 |
| 3 |
Bing, Rachael C (12)
|
VAGATR |
29.69 |
29.77 |
0.08 |
| 4 |
White, Jayla M (12)
|
VASMAC |
30.07 |
30.41 |
0.34 |
| 5 |
Streit, Molly S (12)
|
VACYAC |
31.15 |
30.70 |
-0.45 |
| 6 |
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
31.31 |
30.94 |
-0.37 |
| 7 |
DeSantana-Guidry, Khloe (12)
|
VACYAC |
30.48 |
30.95 |
0.47 |
| 8 |
Miracle, Audrey C (12)
|
VACYAC |
31.65 |
32.39 |
0.74 |
| 9 |
McWhorter, McKenzie M (12)
|
VASMAC |
32.09 |
33.01 |
0.92 |
| 10 |
White, Jakiah I (11)
|
VASMAC |
32.21 |
33.31 |
1.10 |
| 11 |
Macklin, Sophia R (12)
|
VAGATR |
33.27 |
33.45 |
0.18 |
| 12 |
Abdulaeva, Nicole (12)
|
VASMAC |
33.17 |
33.84 |
0.67 |
| 13 |
Davis, Morgan M (11)
|
VAGATR |
36.52 |
34.08 |
-2.44 |
| 14 |
Munday, Madeleine K (12)
|
VAGATR |
35.17 |
34.27 |
-0.90 |
| 15 |
Speicher, Olivia L (11)
|
VASMAC |
33.51 |
34.71 |
1.20 |
| 16 |
Wohlford, Emma T (12)
|
VAGATR |
37.55 |
36.46 |
-1.09 |
| 17 |
Roper, Madeline M (12)
|
VACYAC |
37.64 |
37.95 |
0.31 |
| 18 |
Richardson, Anna K (11)
|
VASMAC |
37.54 |
39.90 |
2.36 |
| 19 |
Campbell, Bailey E (11)
|
VAVOLT |
44.23 |
41.65 |
-2.58 |
| 20 |
Cole, Elsie E (12)
|
VASMAC |
41.46 |
42.58 |
1.12 |
|
Moseley, Kaitlyn S (11)
|
VAGATR |
36.76 |
NS |
- |