| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Jakiah I (14)
|
VASMAC |
29.27 |
27.97 |
-1.30 |
| 2 |
Shafer, Naomi R (13)
|
VAGATR |
27.57 |
28.08 |
0.51 |
| 3 |
Ma, Allison (14)
|
VASMAC |
28.16 |
28.14 |
-0.02 |
| 4 |
Pearson, Catherine I (13)
|
VASMAC |
29.14 |
28.66 |
-0.48 |
| 5 |
Pullen, Alexis L (13)
|
VASMAC |
31.08 |
32.35 |
1.27 |
| 6 |
Sterner, Shayla F (13)
|
VASMAC |
32.92 |
32.71 |
-0.21 |
| 6 |
Kann, Jozelyn F (14)
|
VAGATR |
32.86 |
32.71 |
-0.15 |
| 8 |
Nava Sinay, Rosallyn (13)
|
VASMAC |
41.77 |
34.18 |
-7.59 |
| 9 |
Hartley, Madeline G (13)
|
VASMAC |
36.40 |
35.72 |
-0.68 |
| 10 |
Dempsey, Cathleen (14)
|
VASMAC |
|
44.53 |
- |
|
Hendricksen, Meredith A (13)
|
VAGATR |
|
NS |
- |
|
Mayes, Makenzie B (13)
|
VAGATR |
38.13 |
NS |
- |