| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bonomo, Caroline L (12)
|
NCSMAC |
44.65 |
40.75 |
-3.90 |
| 2 |
Gibson, Jaden (9)
|
NCSMAC |
43.35 |
42.06 |
-1.29 |
| 3 |
Hendershot, Madelyn M (12)
|
NCSMAC |
49.50 |
43.80 |
-5.70 |
| 4 |
Clark, Harper A (12)
|
NCSMAC |
43.73 |
46.00 |
2.27 |
| 5 |
Bonomo, Ava Grace G (10)
|
NCSMAC |
47.08 |
47.17 |
0.09 |
| 6 |
Sherrill, Julia E (9)
|
NCBHRC |
46.00 |
48.68 |
2.68 |
| 7 |
Colvin, Magdalene M (12)
|
NCSMAC |
49.35 |
49.09 |
-0.26 |
| 8 |
McConnell, Molly R (11)
|
NCSMAC |
50.31 |
49.27 |
-1.04 |
| 9 |
McCracken, Lenna K (9)
|
NCSMAC |
59.85 |
54.62 |
-5.23 |
| 10 |
Skinner, Sarah E (11)
|
NCSMAC |
1:03.00 |
59.38 |
-3.62 |
| 11 |
Warlick, Olivia G (8)
|
NCSMAC |
1:00.77 |
1:02.56 |
1.79 |
| 12 |
Cook, Jenna R (9)
|
NCASC |
|
1:07.77 |
- |
|
McCormick, Gillian R (9)
|
NCASC |
|
DQ |
- |
|
Hogan, Cora J (9)
|
NCSMAC |
1:10.64 |
DQ |
- |
|
Kirkpatrick, Melody E (6)
|
NCSMAC |
1:17.81 |
DQ |
- |
|
Sasi, Meghna B (7)
|
NCSMAC |
|
DQ |
- |