| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sanders, Hadley E (10)
|
SEACAC |
47.77 |
44.91 |
-2.86 |
| 2 |
Jenkins, Charlotte F (10)
|
NCAJCC |
51.12 |
50.40 |
-0.72 |
| 3 |
McGowin, Casey E (9)
|
NCBAQ |
1:03.58 |
50.82 |
-12.76 |
| 4 |
Mason, Ella M (9)
|
NCSMAC |
53.75 |
52.68 |
-1.07 |
| 5 |
Hardesty, Sloane (10)
|
NCAJCC |
|
53.36 |
- |
| 6 |
Carr, Emma M (10)
|
NCMHAC |
1:02.41 |
56.81 |
-5.60 |
| 7 |
Springer, Annette J (10)
|
SEACAC |
57.79 |
57.19 |
-0.60 |
| 8 |
Sayler, Meridian E (10)
|
SEACAC |
55.11 |
57.37 |
2.26 |
| 9 |
Beane, Charlotte L (10)
|
NCMHAC |
|
1:03.85 |
- |
| 10 |
Galindo-Gomez, Jayleen A (10)
|
NCSMAC |
1:08.39 |
1:08.01 |
-0.38 |
|
Carver, Isla R (9)
|
NCSMAC |
1:00.40 |
DQ |
- |