| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baley, Ava C (9)
|
NCAJCC |
33.05 |
33.07 |
0.02 |
| 2 |
Savage, Mary Jane M (9)
|
NCAJCC |
35.07 |
34.74 |
-0.33 |
| 3 |
Jenkins, Charlotte F (10)
|
NCAJCC |
36.47 |
35.21 |
-1.26 |
| 4 |
Mason, Ella M (9)
|
NCSMAC |
41.48 |
39.69 |
-1.79 |
| 5 |
McGowin, Casey E (9)
|
NCBAQ |
53.72 |
41.71 |
-12.01 |
| 6 |
Carver, Isla R (9)
|
NCSMAC |
41.86 |
42.03 |
0.17 |
| 7 |
Sosebee, Graceyn (9)
|
NCAJCC |
42.32 |
45.23 |
2.91 |
| 8 |
Lindow, Adeline (9)
|
NCAJCC |
50.58 |
46.90 |
-3.68 |
| 9 |
Sayler, Meridian E (10)
|
SEACAC |
51.15 |
47.54 |
-3.61 |
| 10 |
Garrett, Ella K (9)
|
NCAJCC |
52.34 |
48.33 |
-4.01 |
| 11 |
Beane, Charlotte L (10)
|
NCMHAC |
|
49.22 |
- |
| 12 |
Galindo-Gomez, Jayleen A (10)
|
NCSMAC |
51.56 |
57.50 |
5.94 |
| 13 |
Cole, Jade A (9)
|
NCAJCC |
1:03.01 |
58.10 |
-4.91 |
| 14 |
Springer, Annette J (10)
|
SEACAC |
52.93 |
59.55 |
6.62 |
|
Jimenez, Emma G (9)
|
NCSMAC |
|
DQ |
- |
|
Adams, Emery L (9)
|
NCSMAC |
1:06.28 |
NS |
- |