| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hartley, Nathaniel Q (12)
|
NIFAST |
34.28 |
33.99 |
-0.29 |
| 2 |
Ulatowski, Scarlett D (12)
|
NIFAST |
35.23 |
34.97 |
-0.26 |
| 3 |
Magda, Dylan J (11)
|
NIFAST |
36.29 |
37.65 |
1.36 |
| 4 |
Palmer, Alexander R (11)
|
NIFAST |
40.18 |
38.42 |
-1.76 |
| 5 |
Podzorov, Maya L (10)
|
NIWEBS |
44.54 |
42.01 |
-2.53 |
| 6 |
Uchal, Hannah L (11)
|
NIWEBS |
45.45 |
43.51 |
-1.94 |
| 7 |
Weiner, Lucas M (12)
|
NIFAST |
44.83 |
44.25 |
-0.58 |
| 8 |
Weiner, Nicholas J (12)
|
NIFAST |
49.44 |
44.49 |
-4.95 |
| 9 |
Hinds, Benjamin W (8)
|
NIWEBS |
54.13 |
47.86 |
-6.27 |
| 10 |
Gorton, Claire P (11)
|
NIWEBS |
56.17 |
49.64 |
-6.53 |
| 11 |
Sullivan, Maeve C (10)
|
NIFAST |
58.92 |
55.13 |
-3.79 |
| 12 |
Weiner, Isabella G (9)
|
NIFAST |
|
55.84 |
- |
| 13 |
Conley, Michael T (11)
|
NIFAST |
|
1:01.44 |
- |
|
Huerta-Andrade, Amanda (8)
|
NIFAST |
|
DQ |
- |
|
Oneil, Isabel D (11)
|
NIWEBS |
1:01.94 |
DQ |
- |