| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tenhundfeld, Emma L (12)
|
OHKEY |
2:32.67 |
2:23.41 |
-9.26 |
| 2 |
McCafferty, Katie L (15)
|
OHKEY |
|
2:31.48 |
- |
| 3 |
Kyre, Emily T (12)
|
OHKEY |
2:33.27 |
2:33.07 |
-0.20 |
| 4 |
Harker, Charlie A (14)
|
OHCY |
2:57.81 |
2:36.40 |
-21.41 |
| 5 |
Newkirk, Anna K (15)
|
OHKEY |
|
2:38.61 |
- |
| 6 |
Bernier, Tattiannah I (11)
|
OHSPY |
2:47.22 |
2:38.74 |
-8.48 |
| 7 |
Cowell, Grace M (15)
|
OHKEY |
2:37.40 |
2:38.96 |
1.56 |
| 8 |
Towle, Madelyn G (14)
|
OHKEY |
|
2:39.84 |
- |
| 9 |
Perry, Gabby (14)
|
OHCFY |
|
2:43.53 |
- |
| 10 |
Woltermann, Anna K (16)
|
OHKEY |
2:45.10 |
2:43.73 |
-1.37 |
| 11 |
Mitter, Rachel L (13)
|
OHKEY |
2:48.80 |
2:59.96 |
11.16 |
|
Krug, Madeline B (13)
|
OHKEY |
2:55.87 |
NS |
- |
|
Swedlund, Katherine A (16)
|
OHKEY |
3:02.07 |
NS |
- |