| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sipula, Estella A (11)
|
AMWHAT |
3:09.69 |
2:52.84 |
-16.85 |
| 2 |
Liu, Stacy R (11)
|
AMPTSC |
3:28.19 |
3:00.30 |
-27.89 |
| 3 |
Dunn, Sydney A (12)
|
AMHD |
3:09.68 |
3:02.54 |
-7.14 |
| 4 |
Petrina, Katherine R (12)
|
AMNGAC |
3:25.63 |
3:10.51 |
-15.12 |
| 5 |
Laurenti, Gianna M (11)
|
AMMMSA |
3:15.67 |
3:14.78 |
-0.89 |
| 6 |
Smail, Kendra M (12)
|
AMCSC |
3:34.48 |
3:16.68 |
-17.80 |
| 7 |
Miller, Laurana E (12)
|
AMMMSA |
3:55.01 |
3:16.81 |
-38.20 |
| 8 |
Bowers, Addison R (11)
|
AMPTSC |
3:36.63 |
3:17.35 |
-19.28 |
| 9 |
Alessio, Madison A (12)
|
AMEFSC |
3:20.55 |
3:17.41 |
-3.14 |
| 10 |
Gregorowicz, Abigail S (12)
|
AMPRA |
3:15.67 |
3:17.43 |
1.76 |
| 11 |
Smith, Sydney (11)
|
AMGPAC |
3:25.63 |
3:21.70 |
-3.93 |
| 12 |
Daugherty, Maya J (12)
|
AMHD |
3:16.36 |
3:21.79 |
5.43 |
| 13 |
Race, Delaney M (11)
|
AMMMSA |
3:18.03 |
3:23.25 |
5.22 |
| 14 |
Conwell, Natalie G (12)
|
AMWHAT |
3:29.54 |
3:24.73 |
-4.81 |
| 15 |
Shirley, Caroline B (11)
|
AMFAST |
3:30.68 |
3:24.81 |
-5.87 |
| 16 |
Unadkat, Grishma J (11)
|
AMJCCS |
3:25.19 |
3:27.00 |
1.81 |