| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Porter, Arisa M (10)
|
FGHAT |
44.56 |
36.03 |
-8.53 |
| 2 |
Perry, Francesca R (9)
|
FGSOFL |
42.14 |
37.59 |
-4.55 |
| 3 |
German, Dajah (10)
|
FGSFTL |
42.26 |
41.99 |
-0.27 |
| 4 |
McCranie, Stella (9)
|
FGAZFL |
43.98 |
42.43 |
-1.55 |
| 5 |
Ramraj, Alejandra J (9)
|
FGSUN |
48.79 |
42.78 |
-6.01 |
| 6 |
Rothenburg, Ariel (10)
|
FGTSA |
|
42.96 |
- |
| 7 |
Miller, Rachel E (10)
|
FGSUN |
46.50 |
43.77 |
-2.73 |
| 8 |
Suarez, Ava B (10)
|
FGYSF |
49.73 |
44.66 |
-5.07 |
| 9 |
Cavalcanti, Juliana D (9)
|
FGUN-0 |
45.34 |
45.89 |
0.55 |
| 10 |
Britto, Caitlyn P (10)
|
FGSF |
47.66 |
46.28 |
-1.38 |
| 11 |
Mirones, Lilliana M (9)
|
FGSUN |
49.73 |
46.94 |
-2.79 |
| 12 |
Lopez, Camyla I (10)
|
FGCCPR |
54.26 |
47.99 |
-6.27 |
| 13 |
Jorgensen, Kierstyn E (9)
|
FGSUN |
49.84 |
49.26 |
-0.58 |
| 14 |
Dromgoole, Madison G (10)
|
FGSFTL |
46.90 |
49.32 |
2.42 |
| 15 |
Moen, Aubrey L (9)
|
FGCCPR |
46.65 |
50.63 |
3.98 |
| 16 |
Ramos, Kiara M (10)
|
FGSUN |
|
51.64 |
- |
| 17 |
Reilly, Keira L (10)
|
FGCCPR |
49.57 |
51.83 |
2.26 |
| 18 |
Harding, Aveline (9)
|
FGCSSC |
55.60 |
52.47 |
-3.13 |
| 19 |
Pierre, Abigail (9)
|
FGTSA |
1:04.05 |
52.88 |
-11.17 |
| 20 |
Rodriguez-Quevedo, Alexandra (10)
|
FGSFTL |
48.96 |
53.70 |
4.74 |
| 21 |
Ordonez, Valentina A (9)
|
FGTSA |
1:00.29 |
54.68 |
-5.61 |
| 22 |
Porras, Nahomi C (9)
|
FGAZFL |
1:01.12 |
55.73 |
-5.39 |