| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ng, Trystan S (12)
|
MRCOND |
30.55 |
32.08 |
1.53 |
| 2 |
Negast, Taylor N (12)
|
MRCOND |
32.05 |
32.52 |
0.47 |
| 3 |
Dwyer, Grace V (11)
|
MRCOND |
31.90 |
32.93 |
1.03 |
| 4 |
Levitt, Hannah R (12)
|
CTZEUS |
33.22 |
35.38 |
2.16 |
| 5 |
Anselmi, D'Arcy J (11)
|
MRCOND |
34.71 |
35.53 |
0.82 |
| 6 |
Mejia, Stephani (12)
|
MRYMID |
34.09 |
35.63 |
1.54 |
| 7 |
Balestrieri, Lucia A (11)
|
MRRA |
|
36.21 |
- |
| 8 |
Gazzara, Kaitlyn M (11)
|
MRCOND |
34.72 |
36.64 |
1.92 |
| 9 |
Cedeno, Sofia M (12)
|
MRYMID |
36.64 |
36.78 |
0.14 |
| 10 |
Freeman, Corissa S (11)
|
MRCOND |
36.09 |
38.10 |
2.01 |
| 11 |
Morris Exeter, Maya (11)
|
MRYMID |
38.59 |
38.57 |
-0.02 |
| 12 |
Rabasco, Sarah E (11)
|
MRRA |
38.20 |
38.76 |
0.56 |
| 13 |
Smith, Caroline K (12)
|
MRYMID |
|
40.31 |
- |
| 14 |
Starikovsky, Marina N (12)
|
MRCOND |
40.24 |
40.73 |
0.49 |
| 15 |
Lawson, Olivia C (11)
|
MRCOND |
38.53 |
41.14 |
2.61 |
| 16 |
Serafin, Vivian (11)
|
MRYMID |
43.72 |
43.42 |
-0.30 |
| 17 |
Berard, Elizabeth M (12)
|
MRCOND |
42.90 |
43.56 |
0.66 |
| 18 |
Shpak, Olivia G (12)
|
MRRA |
43.77 |
44.56 |
0.79 |
| 19 |
Venezia, Madelyn S (11)
|
MRRA |
47.53 |
46.32 |
-1.21 |
| 20 |
Lopez, Maria Camila (11)
|
MRYMID |
|
50.95 |
- |
| 21 |
Gottschall, Sydney R (11)
|
MRRA |
|
52.71 |
- |
|
Morgan, Madison R (11)
|
MRRA |
|
DQ |
- |
|
Radick, Susannah (12)
|
MRCFB |
45.25 |
DQ |
- |