| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ha, Kara (12)
|
MRLIFE |
39.38 |
38.81 |
-0.57 |
| 2 |
Clarke, Kristina A (11)
|
MRCAS |
40.65 |
41.75 |
1.10 |
| 3 |
Province, Shaye L (12)
|
MRASAP |
44.02 |
42.89 |
-1.13 |
| 4 |
Almanzar, Libertad (12)
|
MRUN |
44.33 |
44.06 |
-0.27 |
| 5 |
Rodriguez, Isabella D (11)
|
MRKBM |
44.84 |
44.33 |
-0.51 |
| 6 |
Sanchez, Amya (12)
|
MRUN |
44.92 |
44.62 |
-0.30 |
| 7 |
Champagnie, Chanel (12)
|
MRKBM |
46.30 |
46.98 |
0.68 |
| 8 |
Little, Kaylynn D (11)
|
MRKBM |
46.51 |
47.58 |
1.07 |
| 9 |
Wiggins, Alayla (12)
|
MRKBM |
49.52 |
47.84 |
-1.68 |
| 10 |
Plummer, Christina E (11)
|
MRASAP |
49.91 |
49.31 |
-0.60 |
| 11 |
Hidalgo, Ivanka (11)
|
MRUN |
56.78 |
53.85 |
-2.93 |
| 12 |
Valverde, Jahani (11)
|
MRKBM |
1:01.49 |
1:10.11 |
8.62 |