| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dobson, Kennedi (8)
|
MAPENN |
49.62 |
47.79 |
-1.83 |
| 2 |
Cicco, Emily E (8)
|
MAGSA |
55.05 |
49.06 |
-5.99 |
| 3 |
Stevenson, Abigail (8)
|
MAGSA |
55.24 |
50.68 |
-4.56 |
| 4 |
Thoennes, Emma R (8)
|
MAGSA |
|
51.25 |
- |
| 5 |
Radziewicz, Gabriella E (8)
|
MADST |
52.70 |
52.83 |
0.13 |
| 6 |
Napier, Alyssa (8)
|
MAWEST |
1:02.61 |
53.82 |
-8.79 |
| 7 |
Williams, Victoria A (7)
|
MASAKA |
51.85 |
54.16 |
2.31 |
| 8 |
McCullough, Saryn R (8)
|
MAWEST |
1:00.34 |
55.52 |
-4.82 |
| 9 |
Groundwater, Margot G (7)
|
MAFSS |
1:10.20 |
56.67 |
-13.53 |
| 10 |
Ryan, Maxalyn E (7)
|
MADST |
54.54 |
57.32 |
2.78 |
| 11 |
Farag, Hanna (8)
|
MAEAST |
57.35 |
1:01.73 |
4.38 |
| 12 |
Serafin, Gabrielle O (8)
|
MAWEST |
1:03.14 |
1:04.12 |
0.98 |
|
Gotzis, Ella E (8)
|
MAGSA |
|
DQ |
- |
|
Ali, Hanane A (8)
|
MASERP |
|
DQ |
- |
|
Landis, Sylvia A (8)
|
MAFSS |
|
DQ |
- |
|
Mao, Kate J (8)
|
MAGSA |
|
NS |
- |
|
Gounder, Kavya R (8)
|
MAGSA |
|
NS |
- |
|
Shaffer, Naomi R (8)
|
MAPCAC |
|
NS |
- |
|
Burns, Ava L (8)
|
MAGSA |
|
NS |
- |
|
Phillips, Alexandra J (8)
|
MADST |
50.10 |
NS |
- |