| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grossgold, Clara L (8)
|
VATIDE |
40.74 |
40.61 |
-0.13 |
| 2 |
Ballin, Alivia D (8)
|
VA757 |
43.31 |
42.38 |
-0.93 |
| 3 |
Mannato, Bryn K (8)
|
VATIDE |
49.56 |
46.49 |
-3.07 |
| 4 |
Karunaratne, Paige N (8)
|
VATIDE |
55.84 |
46.57 |
-9.27 |
| 5 |
Hamilton, Keller R (8)
|
VA757 |
49.92 |
47.87 |
-2.05 |
| 6 |
Maisenbacher, Amalie K (8)
|
VATIDE |
58.30 |
48.02 |
-10.28 |
| 7 |
Obrien, Reagan K (8)
|
VA757 |
52.09 |
48.31 |
-3.78 |
| 8 |
Stowell, Olivia R (6)
|
VATIDE |
45.68 |
49.15 |
3.47 |
| 9 |
Bradshaw, Piper L (7)
|
VATIDE |
|
51.48 |
- |
| 10 |
McHose, Eleanor (7)
|
VA757 |
51.46 |
52.52 |
1.06 |
| 11 |
Fefferman, Scarlett S (7)
|
VA757 |
51.05 |
54.44 |
3.39 |
| 12 |
Scianandre, Gina G (7)
|
VA757 |
51.88 |
54.63 |
2.75 |
| 13 |
Guheen, Tegan M (6)
|
VATIDE |
1:02.21 |
1:08.55 |
6.34 |
| 14 |
Karunaratne, Olivia C (6)
|
VATIDE |
|
1:09.37 |
- |
| 15 |
Jacobs, Josephine K (6)
|
VA757 |
1:07.34 |
1:10.42 |
3.08 |