| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Page, Benjamin L (14)
|
NCWOW |
23.06 |
23.20 |
0.14 |
| 2 |
Post, Cian D (13)
|
NCWOW |
25.02 |
24.03 |
-0.99 |
| 3 |
Bennison, James W (14)
|
NCWAVE |
24.10 |
24.20 |
0.10 |
| 4 |
Setzer, Sean T (14)
|
NCWOW |
23.68 |
24.43 |
0.75 |
| 5 |
Smith, Zack C (13)
|
NCWAVE |
25.19 |
24.74 |
-0.45 |
| 6 |
Marsteiner, Matthew F (13)
|
NCWAVE |
27.16 |
25.20 |
-1.96 |
| 7 |
Caravaglio, Matthew B (14)
|
NCWOW |
31.78 |
25.21 |
-6.57 |
| 8 |
Langtry, Ryan C (14)
|
NCWOW |
24.93 |
25.27 |
0.34 |
| 9 |
Hurlbert, Cole R (14)
|
NCWAVE |
25.57 |
25.80 |
0.23 |
| 10 |
Stuck, Evan J (14)
|
NCWOW |
26.37 |
26.43 |
0.06 |
| 11 |
Hughes, Jackson C (13)
|
NCWOW |
25.74 |
26.54 |
0.80 |
| 12 |
Hutchins, Matthew T (13)
|
NCWAVE |
27.28 |
27.10 |
-0.18 |
| 13 |
Milburn-Pyle, James (14)
|
NCWAVE |
28.44 |
27.19 |
-1.25 |
| 14 |
Eberhardt, Nathan L (14)
|
NCWOW |
27.85 |
27.77 |
-0.08 |
| 15 |
Hall, Jackson H (14)
|
NCWAVE |
28.86 |
29.26 |
0.40 |
| 16 |
Page, Logan A (13)
|
NCWAVE |
33.64 |
30.68 |
-2.96 |
| 17 |
Chen, Jinghao (13)
|
NCWAVE |
|
33.79 |
- |