| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Furbay, Caroline D (13)
|
NCBLUE |
25.31 |
25.10 |
-0.21 |
| 2 |
Lac, Tabitha Y (14)
|
NCBLAK |
26.51 |
27.07 |
0.56 |
| 3 |
Murtagh, Leah P (14)
|
NCBLAK |
27.09 |
27.48 |
0.39 |
| 3 |
Pierson, Sarah S (13)
|
NCBLUE |
27.40 |
27.48 |
0.08 |
| 5 |
Weigand, Hailey J (14)
|
NCBLAK |
27.57 |
28.31 |
0.74 |
| 6 |
Williams, Lexi M (13)
|
NCBLAK |
28.33 |
28.83 |
0.50 |
| 7 |
Hubard, Lucinda R (14)
|
NCBLAK |
28.42 |
28.84 |
0.42 |
| 8 |
McQueen, Elizabeth P (14)
|
NCBLUE |
28.29 |
29.29 |
1.00 |
| 9 |
Burke, Marissa N (13)
|
NCBLUE |
30.32 |
31.64 |
1.32 |
| 10 |
Bongkotmanee, Ida P (13)
|
NCBLAK |
30.87 |
31.85 |
0.98 |
|
Hawthorne, Morgan A (14)
|
NCBLUE |
26.66 |
NS |
- |
|
Litton, Maegan Grace K (13)
|
NCBLUE |
33.34 |
NS |
- |