| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hastings, Lucy (8)
|
NCWOW |
25.96 |
25.92 |
-0.04 |
| 2 |
Senter, Ainsley J (7)
|
NCCFAC |
27.15 |
27.10 |
-0.05 |
| 3 |
House, Asheford R (8)
|
NCCFAC |
28.87 |
28.69 |
-0.18 |
| 4 |
Spencer, Summer R (8)
|
NCCFAC |
33.77 |
29.54 |
-4.23 |
| 5 |
Herring, Summer S (7)
|
NCBAC |
36.25 |
31.57 |
-4.68 |
| 6 |
Cumbee, Phoebe A (8)
|
NCBAC |
|
32.79 |
- |
| 7 |
Carroll, Charlotte A (8)
|
NCWOW |
30.05 |
33.35 |
3.30 |
| 8 |
Witmer, Gentry L (6)
|
NCCFAC |
29.88 |
33.80 |
3.92 |
|
McMullan, Annabelle B (4)
|
NCBAC |
|
DQ |
- |
|
Nester, Addison S (7)
|
NCBAC |
38.83 |
DQ |
- |
|
Hohl, Ava M (7)
|
NCCFAC |
|
DQ |
- |
|
Garver, Meghan E (8)
|
NCBAC |
|
NS |
- |
|
Stegman, Peyton N (8)
|
NCBAC |
30.43 |
NS |
- |