| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grill, Jenna M (15)
|
NCWOW |
59.91 |
59.65 |
-0.26 |
| 2 |
Goldblum, McKaley Q (17)
|
NCWOW |
1:05.20 |
1:01.36 |
-3.84 |
| 3 |
Joy, Madison A (13)
|
NCWOW |
1:03.88 |
1:03.99 |
0.11 |
| 4 |
Furbay, Caroline D (13)
|
UNAT |
1:06.21 |
1:04.87 |
-1.34 |
| 5 |
Ginsberg, Kinsey A (15)
|
NCWOW |
1:03.18 |
1:05.34 |
2.16 |
| 6 |
Bazinet, Laurie Anne (17)
|
NCWOW |
1:01.07 |
1:05.91 |
4.84 |
| 7 |
Hawthorne, Morgan A (14)
|
NCWOW |
1:04.44 |
1:05.96 |
1.52 |
| 8 |
Lac, Tabitha Y (14)
|
NCWOW |
1:10.26 |
1:06.61 |
-3.65 |
| 9 |
Bishop, Avery M (15)
|
NCWOW |
1:06.28 |
1:08.14 |
1.86 |
| 10 |
Johnson, Emma C (16)
|
NCWOW |
1:10.61 |
1:08.75 |
-1.86 |
| 11 |
Hyldahl, Aubrey E (18)
|
NCWOW |
1:06.60 |
1:10.58 |
3.98 |
| 12 |
Hoover, Madison M (17)
|
NCWOW |
1:02.20 |
1:10.74 |
8.54 |
| 13 |
Fairchild, Abigail V (17)
|
NCWOW |
1:08.07 |
1:11.47 |
3.40 |
| 14 |
Kugatow, Bobbie L (13)
|
NCWOW |
1:17.26 |
1:12.34 |
-4.92 |
| 15 |
McQueen, Elizabeth P (14)
|
NCWOW |
1:16.18 |
1:12.44 |
-3.74 |
| 16 |
Gerock, Austin M (14)
|
NCWOW |
1:13.22 |
1:12.72 |
-0.50 |
| 17 |
Pierson, Sarah S (13)
|
NCWOW |
1:17.68 |
1:13.54 |
-4.14 |
| 18 |
Hughes, Bailey L (15)
|
NCWOW |
1:09.60 |
1:14.03 |
4.43 |
| 19 |
Hannum, Ellie G (17)
|
NCWOW |
1:09.13 |
1:14.55 |
5.42 |
| 20 |
Howard, Maren W (16)
|
NCWOW |
1:12.19 |
1:14.81 |
2.62 |