| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCarthy, Luke T (12)
|
NCECA |
26.67 |
26.73 |
0.06 |
| 2 |
Hughes, Jackson C (12)
|
NCWOW |
26.88 |
27.11 |
0.23 |
| 2 |
Camacho, Clement D (11)
|
NCUN |
27.36 |
27.11 |
-0.25 |
| 4 |
Zapler, Ezra N (11)
|
NCECA |
27.07 |
27.18 |
0.11 |
| 5 |
Hawthorne, Maxwell S (11)
|
NCWOW |
28.71 |
28.84 |
0.13 |
| 6 |
Cole, Tyrus D (12)
|
NCECA |
28.60 |
29.02 |
0.42 |
| 7 |
Arredondo, Nathan B (11)
|
NCWOW |
29.67 |
29.46 |
-0.21 |
| 8 |
Reiniche, Ryan J (11)
|
NCWOW |
30.21 |
29.49 |
-0.72 |
| 9 |
Cook, Tyson G (12)
|
NCECA |
31.27 |
30.37 |
-0.90 |
| 10 |
Tillery, Wade H (12)
|
NCWOW |
30.55 |
31.04 |
0.49 |
| 11 |
Marlow, Tiago V (12)
|
NCECA |
30.75 |
31.13 |
0.38 |
| 12 |
Park, Dylan T (11)
|
NCWOW |
31.82 |
31.67 |
-0.15 |
| 13 |
House, William E (11)
|
NCUN |
|
31.92 |
- |
| 14 |
Pesko, Gavin E (11)
|
NCECA |
32.93 |
32.53 |
-0.40 |
| 15 |
Ive, Jethro T (12)
|
NCWOW |
33.42 |
32.77 |
-0.65 |
| 16 |
Healey, Matthew J (11)
|
NCWOW |
33.69 |
32.93 |
-0.76 |
| 17 |
Bollenbacher, Stephen W (11)
|
NCWOW |
33.23 |
34.22 |
0.99 |
| 18 |
Finch, Aidan J (12)
|
NCECA |
34.09 |
35.75 |
1.66 |
| 19 |
Blaylock, Dakota M (11)
|
NCECA |
39.42 |
37.17 |
-2.25 |