| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Metzinger, Mya Z (11)
|
NCWOW |
42.35 |
41.34 |
-1.01 |
| 2 |
Milks, Bailey A (12)
|
NCWOW |
43.70 |
42.32 |
-1.38 |
| 3 |
Hughes, Chloe A (11)
|
NCWOW |
43.81 |
42.94 |
-0.87 |
| 4 |
Burke, Marissa N (12)
|
NCWOW |
42.84 |
44.85 |
2.01 |
| 5 |
Norris, Chloe L (12)
|
NCWOW |
44.19 |
45.67 |
1.48 |
| 6 |
Balderson, Cameron G (12)
|
NCWOW |
49.75 |
47.73 |
-2.02 |
| 7 |
Phillips, Sarah C (9)
|
NCWOW |
47.05 |
47.87 |
0.82 |
| 8 |
Carroll, Mackenzie M (10)
|
NCWOW |
49.27 |
48.99 |
-0.28 |
| 9 |
McElroy, Sydney K (9)
|
NCWOW |
50.57 |
49.44 |
-1.13 |
| 10 |
Uebbing, CoCo A (10)
|
NCWOW |
48.85 |
49.79 |
0.94 |
| 11 |
Sterner, Adalyn G (11)
|
NCWOW |
53.82 |
52.76 |
-1.06 |
| 12 |
Zimmerman, Mary C (10)
|
NCWOW |
56.50 |
54.71 |
-1.79 |
| 13 |
Hastings, Lucy (8)
|
NCWOW |
54.41 |
55.70 |
1.29 |
| 14 |
Harrington, Jordan T (9)
|
NCWOW |
56.64 |
55.98 |
-0.66 |
| 15 |
Strickland, Lyla M (9)
|
NCWOW |
53.94 |
56.03 |
2.09 |
| 16 |
Baynard, Melina K (9)
|
NCWOW |
51.32 |
56.24 |
4.92 |
| 17 |
Ezell-Tapia, Tiegan D (10)
|
NCWOW |
|
1:00.41 |
- |
| 18 |
Steele, Isabel C (9)
|
NCWOW |
1:03.66 |
1:05.21 |
1.55 |
| 19 |
Carroll, Charlotte A (8)
|
NCWOW |
1:01.86 |
1:06.04 |
4.18 |
| 20 |
McLean, Claire R (7)
|
NCWOW |
1:07.54 |
1:06.70 |
-0.84 |
| 21 |
Van Nostrand, Shea A (10)
|
NCWOW |
1:16.45 |
1:07.32 |
-9.13 |
| 22 |
DeSelms, Stella R (8)
|
NCWOW |
|
1:08.64 |
- |
|
Savage, Margaret C (7)
|
NCWOW |
1:01.22 |
DQ |
- |
|
Nicholson, Madelyn G (8)
|
NCWOW |
55.94 |
NS |
- |