| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wile, Tripp V (10)
|
VATSU |
2:38.33 |
2:43.17 |
4.84 |
| 2 |
Acholonu, Arinzechukwu M (10)
|
PVNCAP |
3:02.07 |
2:56.20 |
-5.87 |
| 3 |
Shafer, William H (10)
|
VAWST |
3:19.38 |
2:59.37 |
-20.01 |
| 4 |
Kibler, Liam D (9)
|
VABASS |
2:53.67 |
3:01.73 |
8.06 |
| 5 |
Stanley, Spencer W (10)
|
MDMAC |
3:09.97 |
3:04.87 |
-5.10 |
| 6 |
Schroeder, Austin (10)
|
PVNCAP |
3:10.11 |
3:07.17 |
-2.94 |
| 7 |
Branch, Samuel C (9)
|
MDMAC |
3:05.81 |
3:07.28 |
1.47 |
| 8 |
Smith, Jackson T (9)
|
MDFSC |
3:15.37 |
3:07.29 |
-8.08 |
| 9 |
Scott, Dylan C (10)
|
MDFSC |
3:20.94 |
3:15.53 |
-5.41 |
| 10 |
Speicher, Ulrich M (10)
|
MDMAC |
3:12.39 |
3:17.62 |
5.23 |
| 11 |
Zheng, Logan J (9)
|
MDMAC |
3:21.90 |
3:17.68 |
-4.22 |
| 12 |
Hess, Sebastian R (8)
|
MDCGA |
3:18.66 |
3:23.41 |
4.75 |
| 13 |
Patten, Brandon C (9)
|
PVNCAP |
3:17.58 |
3:23.84 |
6.26 |
| 14 |
Stambaugh, Jennings E (10)
|
MDMAC |
3:31.97 |
3:34.19 |
2.22 |
| 15 |
Smith, Avery M (10)
|
AMBRY |
3:12.95 |
3:35.23 |
22.28 |
| 16 |
Ayala, Jayden I (10)
|
VAWST |
3:28.12 |
3:36.05 |
7.93 |
| 17 |
Abraham, Mitchell P (9)
|
PVNCAP |
3:22.68 |
3:37.24 |
14.56 |
| 18 |
Nevares Gonzalez de, Alberto (8)
|
MDMAC |
3:46.00 |
3:38.83 |
-7.17 |
| 19 |
Kundey, Rojan L (7)
|
MDMAC |
4:03.93 |
3:39.95 |
-23.98 |
| 20 |
Baker, Bennett N (10)
|
MDMAC |
4:30.55 |
3:42.02 |
-48.53 |
| 21 |
Marshall, Connor J (9)
|
MDCGA |
3:54.87 |
3:45.21 |
-9.66 |
| 22 |
Gonzalez, Roman L (9)
|
MDMAC |
4:00.00 |
3:45.68 |
-14.32 |
| 23 |
Johnson, Liam A (8)
|
VAWST |
3:52.76 |
3:48.34 |
-4.42 |
| 24 |
Zhou, Jeffrey (10)
|
MDMAC |
3:59.08 |
3:52.95 |
-6.13 |
| 25 |
Cote, Luke R (9)
|
VAWST |
4:01.42 |
3:53.81 |
-7.61 |
| 26 |
McClure, Reece G (9)
|
MDMAC |
3:58.00 |
3:57.64 |
-0.36 |
| 27 |
Zhou, Ray L (9)
|
MDMAC |
4:36.00 |
4:32.47 |
-3.53 |