| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wiles, Morgan E (12)
|
NCTYDE |
28.62 |
28.30 |
-0.32 |
| 2 |
Frondoza, Audrianne R (12)
|
NCTYDE |
29.06 |
29.02 |
-0.04 |
| 3 |
Lewis, Lauren E (11)
|
NCTYDE |
30.53 |
29.78 |
-0.75 |
| 3 |
Welborn, Sophia S (11)
|
NCTYDE |
30.15 |
29.78 |
-0.37 |
| 5 |
High, Mary D (11)
|
NCTYDE |
32.63 |
30.23 |
-2.40 |
| 6 |
Mabe, Molly K (12)
|
NCTYDE |
30.13 |
30.59 |
0.46 |
| 7 |
Granger, Olivia L (12)
|
NCTYDE |
30.56 |
31.01 |
0.45 |
| 9 |
Steele, Ava C (11)
|
NCTYDE |
34.55 |
31.68 |
-2.87 |
| 11 |
Post, Alejandra M (11)
|
NCTYDE |
33.21 |
33.26 |
0.05 |
| 12 |
Parks, Bronwyn E (11)
|
NCTYDE |
35.48 |
33.37 |
-2.11 |
| 13 |
Whitworth, Margaret E (12)
|
NCTYDE |
34.45 |
33.38 |
-1.07 |
| 15 |
Maxey, Mallory E (11)
|
NCTYDE |
37.47 |
34.87 |
-2.60 |
| 21 |
Lewis, Piper A (11)
|
NCTYDE |
40.23 |
38.09 |
-2.14 |