| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arciniegas, Alessandra (13)
|
MRCOND |
25.60 |
25.68 |
0.08 |
| 2 |
Grayson, Kelsey R (13)
|
MRBGNW |
25.95 |
26.22 |
0.27 |
| 3 |
Cattanach, Clara (13)
|
MRBGNW |
26.16 |
26.61 |
0.45 |
| 4 |
Lovig, Natalie J (13)
|
MRBGNW |
26.07 |
26.64 |
0.57 |
| 5 |
Boda, Zoe M (13)
|
MRCOND |
27.94 |
27.25 |
-0.69 |
| 6 |
Zellner-Portela, Natasha N (13)
|
MRNYS |
27.40 |
27.36 |
-0.04 |
| 7 |
White, Finley M (13)
|
MRBGNW |
27.52 |
27.47 |
-0.05 |
| 8 |
Mackof, Delilah N (13)
|
MRBGNW |
28.48 |
28.25 |
-0.23 |
| 9 |
Reed, Claire H (13)
|
MRBAD |
29.52 |
28.28 |
-1.24 |
| 10 |
Oh, Madeline J (13)
|
MRBGNW |
30.82 |
28.94 |
-1.88 |
| 11 |
Del Castillo, Victoria (13)
|
MRCOND |
|
30.71 |
- |
| 12 |
Pothen, Ruth S (13)
|
MRCOND |
31.80 |
30.75 |
-1.05 |
| 13 |
Liu, Natalie (13)
|
MRWEST |
34.86 |
31.96 |
-2.90 |
| 14 |
Krupp, Sophie (13)
|
MRCOND |
32.32 |
32.49 |
0.17 |
| 15 |
Ferrara, Olivia A (13)
|
MRCOND |
31.61 |
32.66 |
1.05 |
| 16 |
Begolli, Rea (13)
|
MRCOND |
37.24 |
36.89 |
-0.35 |