| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harnish, Meaghan K (12)
|
MAYY |
2:28.42 |
2:27.68 |
-0.74 |
| 2 |
Smith, Amber M (12)
|
MDBAAC |
2:39.89 |
2:36.89 |
-3.00 |
| 3 |
Leydig, Camryn G (12)
|
MAYY |
2:59.99 |
2:48.21 |
-11.78 |
| 4 |
Kapp, Christine O (12)
|
MAYY |
2:56.41 |
2:49.55 |
-6.86 |
| 5 |
McNerney, Taylor J (12)
|
MDNBAC |
2:52.48 |
2:49.64 |
-2.84 |
| 6 |
Paxton, Madelyn L (11)
|
MAYY |
2:56.14 |
2:51.04 |
-5.10 |
| 7 |
Kim, Min Joo X (12)
|
MDNBAC |
2:52.97 |
2:52.20 |
-0.77 |
| 8 |
McKenna, Eliza R (10)
|
MDNBAC |
3:09.26 |
3:00.85 |
-8.41 |
| 9 |
Behling, Clara A (10)
|
MAFCA |
3:08.89 |
3:00.88 |
-8.01 |
| 10 |
Pektor, Ava G (11)
|
MAUN |
3:05.99 |
3:03.59 |
-2.40 |
| 11 |
Fetter, Hannah G (12)
|
MACYA |
3:16.03 |
3:09.16 |
-6.87 |
| 12 |
Waldt, Madeline E (12)
|
MDBAAC |
3:28.67 |
3:16.18 |
-12.49 |
| 13 |
Burson, Amaya S (11)
|
MAFCA |
3:11.93 |
3:21.07 |
9.14 |
| 14 |
Honadel, Erica L (12)
|
MDBAAC |
3:18.53 |
3:22.76 |
4.23 |
| 15 |
Richardson, Jesse J (12)
|
MACYA |
3:36.52 |
3:27.11 |
-9.41 |
| 16 |
Hall, Alexandra E (11)
|
MDACSS |
|
3:31.44 |
- |
| 17 |
Watson, Georgia N (11)
|
MDACSS |
|
3:37.74 |
- |
| 18 |
Wang, Margaret S (11)
|
MDBAAC |
|
3:38.28 |
- |
| 19 |
Fernandes, Leah S (10)
|
MAFCA |
|
3:40.25 |
- |
| 20 |
Pasquinelli, Lillian R (11)
|
MDACSS |
|
3:43.10 |
- |
|
Ketterman, Katelynn C (12)
|
MAGETY |
|
3:05.60 |
- |