| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rockett, Alexandra H (19)
|
INIU |
51.81 |
51.14 |
-0.67 |
| 2 |
Heilbrun, Caroline R (17)
|
PVFISH |
52.33 |
51.73 |
-0.60 |
| 3 |
O'Rourke, Kathleen M (18)
|
MRCOL |
52.14 |
51.93 |
-0.21 |
| 4 |
Curry, Camryn C (15)
|
VAPSDN |
51.60 |
51.97 |
0.37 |
| 5 |
Seiberlich, Emma W (15)
|
MAPAY |
52.26 |
52.15 |
-0.11 |
| 6 |
Chew, Lauren E (21)
|
NCECU |
52.36 |
52.28 |
-0.08 |
| 7 |
Magnuson, Kjirsten A (21)
|
CALMU |
51.35 |
52.60 |
1.25 |
| 8 |
Drew, Paxtyn D (19)
|
OHUN-0 |
53.70 |
52.66 |
-1.04 |
| 9 |
Soule, Lise (24)
|
CALMU |
52.70 |
52.71 |
0.01 |
| 10 |
Duggan, Katie A (21)
|
MAUN-1 |
51.99 |
52.92 |
0.93 |
| 11 |
Bradford, Madeline M (20)
|
INIUPU |
52.70 |
53.24 |
0.54 |
| 12 |
Vanlandeghem, Tia J (18)
|
AMPITT |
53.21 |
53.39 |
0.18 |
| 13 |
Oleksiak, Jennifer J (21)
|
INIUPU |
53.10 |
53.57 |
0.47 |
| 14 |
Poliseno, Taylor N (19)
|
NEBRYU |
54.50 |
55.96 |
1.46 |
|
Pfeifer, Genevieve M (15)
|
OZFAST |
51.89 |
DQ |
- |