| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walsh, Alexandra (12)
|
CTCT |
24.38 |
23.97 |
-0.41 |
| 2 |
Mack, Katelyn G (12)
|
PVPV |
24.44 |
24.60 |
0.16 |
| 3 |
Regenauer, Christiana M (12)
|
MRMETR |
24.28 |
24.91 |
0.63 |
| 4 |
Competello, Madeline G (12)
|
MRMETR |
25.26 |
25.10 |
-0.16 |
| 5 |
Famous, Caroline A (12)
|
MAMA |
24.90 |
25.21 |
0.31 |
| 6 |
Landeryou, Sophia A (11)
|
PVPV |
25.39 |
25.25 |
-0.14 |
| 7 |
Harter, Abigail E (12)
|
PVPV |
25.30 |
25.35 |
0.05 |
| 8 |
Tuttle, Mackenzie M (12)
|
MRMETR |
25.19 |
25.46 |
0.27 |
| 9 |
Chiu, Stephanie (12)
|
NJNJS |
25.59 |
25.50 |
-0.09 |
| 10 |
Ramey, Abigail C (12)
|
AMAM |
25.29 |
25.60 |
0.31 |
| 11 |
Paglia, Sydney J (12)
|
MAMA |
25.24 |
25.63 |
0.39 |
| 12 |
Haluszczak, Macrae A (12)
|
AMAM |
25.88 |
25.91 |
0.03 |
| 13 |
Opuszynski, Allison (12)
|
CTCT |
25.79 |
25.97 |
0.18 |
| 14 |
Brinker, Mary E (12)
|
NINI |
25.98 |
26.11 |
0.13 |
| 15 |
Ross, Leia (12)
|
AMAM |
26.18 |
26.34 |
0.16 |
| 16 |
Saliba, Victoria A (12)
|
NJNJS |
25.58 |
26.47 |
0.89 |
| 17 |
Raab, Shannon C (12)
|
MAMA |
25.51 |
26.73 |
1.22 |
| 18 |
McCune, Allison G (12)
|
NINI |
26.50 |
26.77 |
0.27 |
| 19 |
Tarantola, Grace J (12)
|
NJNJS |
26.03 |
26.92 |
0.89 |
| 20 |
Campbell, Hope C (12)
|
MEMAIN |
26.93 |
26.99 |
0.06 |
| 21 |
Williams, Mattie B (12)
|
ADAD |
27.47 |
27.00 |
-0.47 |
| 22 |
Gordon, Jessica E (12)
|
NINI |
26.55 |
27.25 |
0.70 |
| 23 |
Hamilton, Sidney C (12)
|
MEMAIN |
26.67 |
27.52 |
0.85 |
| 24 |
Dybowski, Kaitlyn D (12)
|
ADAD |
27.82 |
27.84 |
0.02 |
| 25 |
Digiovanni, Amaya M (11)
|
ADAD |
27.94 |
28.07 |
0.13 |
| 26 |
Mahoney, Caroline E (12)
|
MEMAIN |
27.35 |
28.13 |
0.78 |
| 27 |
Doughty, Emma J (12)
|
NINI |
29.15 |
29.10 |
-0.05 |
|
Scott, Samantha (12)
|
CTCT |
25.61 |
NS |
- |