| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DelaCruz, Jacob E (10)
|
GUCFSC |
41.72 |
39.37 |
-2.35 |
| 2 |
Kumar, Raoul G (9)
|
GU300 |
40.40 |
39.53 |
-0.87 |
| 3 |
Williams, Evan S (9)
|
GUCFSC |
45.12 |
40.99 |
-4.13 |
| 4 |
Curran, Max F (9)
|
GUVSST |
46.06 |
41.06 |
-5.00 |
| 5 |
Mauricio, Dean (9)
|
GUCFSC |
46.33 |
43.05 |
-3.28 |
| 6 |
Nguyen, Kerry (10)
|
GUCFSC |
49.61 |
43.42 |
-6.19 |
| 7 |
Allen, Charles T (10)
|
GU300 |
54.14 |
44.61 |
-9.53 |
| 8 |
Lemp, Charlton G (9)
|
GUCFSC |
46.58 |
44.62 |
-1.96 |
| 9 |
Speedie, Adam J (10)
|
GUVSST |
|
45.18 |
- |
| 10 |
Fontenette, Jackson J (10)
|
GUCFSC |
45.65 |
46.07 |
0.42 |
| 11 |
Alcala, Aiden J (10)
|
GUCFSC |
47.04 |
46.65 |
-0.39 |
| 12 |
Revelas, Nicholas W (9)
|
GUVSST |
49.32 |
46.72 |
-2.60 |
| 13 |
Leopold, Leo J (9)
|
GUCFSC |
52.77 |
48.09 |
-4.68 |
| 14 |
Li, Fangyuan (9)
|
GUKATY |
44.17 |
48.21 |
4.04 |
| 15 |
Tink, Joshua E (10)
|
GUUSA |
1:00.05 |
48.54 |
-11.51 |
| 16 |
Panek, Grayson A (9)
|
GUCFSC |
56.01 |
48.66 |
-7.35 |
| 17 |
Wiesenborn, Carson S (10)
|
GUKATY |
45.67 |
49.26 |
3.59 |
| 18 |
Chatterjee, Reuben (10)
|
GUCFSC |
55.88 |
51.86 |
-4.02 |
| 19 |
Stoebner, Brandon M (10)
|
GUVSST |
|
52.14 |
- |
| 20 |
Waterman, Luke A (9)
|
GUCFSC |
1:00.49 |
53.07 |
-7.42 |
| 21 |
Cavazos, Luca A (9)
|
GUCFSC |
59.33 |
55.79 |
-3.54 |
| 22 |
Kim, Taevin (9)
|
GUCFSC |
1:02.88 |
57.79 |
-5.09 |
| 23 |
Cavazos, Joaquin G (10)
|
GUCFSC |
48.83 |
57.91 |
9.08 |
| 24 |
Schuchardt, Zachary A (10)
|
GUCFSC |
1:00.42 |
1:05.94 |
5.52 |
|
Metcalf, Aidan c (9)
|
GUEPRC |
1:17.80 |
DQ |
- |
|
Pan, Daniel (9)
|
GU300 |
|
NS |
- |
|
Tran, Minh H (10)
|
GUCFSC |
|
NS |
- |
|
Ngotran, Zachary D (10)
|
GUCFSC |
|
NS |
- |
|
Choi-Kim, William C (9)
|
GUCFSC |
1:03.86 |
NS |
- |