| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smoker, Ella J (13)
|
GUCFSC |
2:28.46 |
2:29.91 |
1.45 |
| 2 |
Cox, Gracelyn C (14)
|
GUKATY |
2:28.23 |
2:37.02 |
8.79 |
| 3 |
Miller, Hayden K (14)
|
GUCFSC |
2:37.49 |
2:37.34 |
-0.15 |
| 4 |
Yao, Lauren (14)
|
GUKATY |
2:42.47 |
2:40.55 |
-1.92 |
| 5 |
Bazzi, Athena J (13)
|
GUKATY |
2:47.32 |
2:41.18 |
-6.14 |
| 6 |
Osorio, Maria V (13)
|
GUCFSC |
2:48.12 |
2:44.43 |
-3.69 |
| 7 |
Craft, Taylor L (13)
|
GUKATY |
2:52.08 |
2:44.46 |
-7.62 |
| 8 |
Goers, Apple L (13)
|
GUCFSC |
2:46.69 |
2:47.39 |
0.70 |
| 9 |
Dillon, Kynley R (13)
|
GUKATY |
2:54.87 |
2:49.85 |
-5.02 |
| 10 |
Devens, Ellie V (13)
|
GUCFSC |
3:15.76 |
2:51.41 |
-24.35 |
| 11 |
Bronte, Zoe N (13)
|
GULSST |
2:55.66 |
2:53.44 |
-2.22 |
| 12 |
Dao, Venus C (13)
|
GUCFSC |
2:54.40 |
2:57.77 |
3.37 |
| 13 |
See, Valerie Y (14)
|
GUKATY |
2:45.09 |
2:58.33 |
13.24 |
| 14 |
Ram, Ellory K (13)
|
GUKATY |
|
2:59.64 |
- |
| 15 |
Pulkkinen, Josephine O (13)
|
GUKATY |
|
3:01.58 |
- |
| 16 |
Rudberg, Madison B (14)
|
GUKATY |
3:09.22 |
3:12.26 |
3.04 |
| 17 |
Reuber, Allison S (13)
|
GUKATY |
|
3:12.59 |
- |
| 18 |
Landin-Seneriz, Adriana (13)
|
GUKATY |
3:22.28 |
3:14.16 |
-8.12 |
|
Mok, Samantha H (14)
|
GUKATY |
2:45.22 |
DQ |
- |