| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coakley, William J (17)
|
GUKATY |
46.73 |
50.02 |
3.29 |
| 2 |
Mitchell, Jake (17)
|
GUKATY |
48.84 |
50.24 |
1.40 |
| 3 |
Cooke, Chase A (16)
|
GUKATY |
48.97 |
50.27 |
1.30 |
| 4 |
Liao, Justin J (15)
|
GUKATY |
53.24 |
50.77 |
-2.47 |
| 5 |
Washington, Demarcus D (15)
|
GUKATY |
51.36 |
52.37 |
1.01 |
| 6 |
Alvarez, Kevin A (17)
|
GUKATY |
50.48 |
53.22 |
2.74 |
| 7 |
Sippel, Ben R (15)
|
GUKATY |
55.37 |
54.34 |
-1.03 |
| 8 |
Mujahed, Youssof E (15)
|
GUKATY |
52.97 |
54.36 |
1.39 |
| 9 |
Martin, Sean A (16)
|
GUKATY |
56.06 |
55.03 |
-1.03 |
| 10 |
Jasman, Ethan P (16)
|
GUKATY |
53.70 |
55.12 |
1.42 |
| 11 |
Riede Noriega, Andre D (15)
|
GUKATY |
|
56.64 |
- |
| 12 |
Riede, Derek A (16)
|
GUKATY |
|
58.92 |
- |
|
Song, Yejun (16)
|
GUKATY |
51.39 |
NS |
- |