| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wells, Amelia S (8)
|
GUKATY |
48.38 |
48.35 |
-0.03 |
| 2 |
Poteet, Alexandra A (8)
|
GUECS |
|
48.38 |
- |
| 3 |
Kolomytseva, Diana L (8)
|
GUECS |
|
49.01 |
- |
| 4 |
Masch, Bianca D (8)
|
GUKATY |
54.09 |
49.74 |
-4.35 |
| 5 |
Fults, Savannah E (8)
|
GUKATY |
53.55 |
54.68 |
1.13 |
| 6 |
Mansingka, Anaya N (8)
|
GUKATY |
1:24.15 |
57.00 |
-27.15 |
| 7 |
Judd, Veronica G (8)
|
GUKATY |
|
1:03.63 |
- |
| 8 |
Schenk, Mia S (8)
|
GUECS |
|
1:12.62 |
- |
|
Harms, Audrey H (8)
|
GUKATY |
51.07 |
DQ |
- |
|
Gonzalez Paisley, Evita L (8)
|
GUKATY |
|
DQ |
- |
|
Ewers, Adley E (8)
|
GUPLAT |
|
DQ |
- |
|
McCain, Kristen M (8)
|
GUKATY |
57.78 |
NS |
- |