| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crossland, Kannen L (10)
|
GUUN-M |
41.97 |
36.07 |
-5.90 |
| 2 |
Schmitt, Carter (9)
|
GUSSAN |
38.35 |
37.27 |
-1.08 |
| 3 |
Simmons, Tate C (9)
|
GUMAC |
40.21 |
37.80 |
-2.41 |
| 4 |
Biddlecome, Alexander R (10)
|
GUMARC |
38.30 |
39.50 |
1.20 |
| 5 |
Petersen, Vincent R (10)
|
GUSSAN |
40.22 |
39.83 |
-0.39 |
| 6 |
Otwell, Karson D (9)
|
GUSSAN |
40.95 |
40.68 |
-0.27 |
| 7 |
Merriken, Wyatt L (9)
|
GUMAC |
45.64 |
40.96 |
-4.68 |
| 8 |
Ferguson, Julius R (9)
|
GUMAC |
40.36 |
41.72 |
1.36 |
| 9 |
Crenshaw, Holland J (10)
|
GUUN-S |
40.88 |
41.76 |
0.88 |
| 10 |
Matula, Adam P (10)
|
GUMAC |
46.80 |
45.99 |
-0.81 |
| 11 |
Heinsohn, Connor Z (9)
|
GUSSAN |
44.89 |
47.12 |
2.23 |
| 12 |
Nixon, Ethan J (9)
|
GUSSAN |
46.60 |
48.07 |
1.47 |
| 13 |
Alarcon, Samuel D (10)
|
GUSSAN |
47.21 |
48.35 |
1.14 |
| 14 |
Stephens, Jack E (9)
|
GUSSAN |
50.10 |
48.73 |
-1.37 |
| 15 |
Henderson, Landon T (9)
|
GUSSAN |
|
50.37 |
- |
| 16 |
Loeffel, Samuel S (9)
|
GUSSAN |
59.97 |
51.17 |
-8.80 |
| 17 |
Gonzalez, Alejandro (9)
|
GUMAC |
52.92 |
55.29 |
2.37 |
|
Crabtree, Nicholas D (10)
|
GUMAC |
1:15.48 |
DQ |
- |
|
Lee, Jeremy M (10)
|
GUSSAN |
53.85 |
NS |
- |
|
San Miguel, Thomas K (10)
|
GUMARC |
|
NS |
- |
|
San Miguel, Landry W (10)
|
GUMARC |
|
NS |
- |