| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Criscione, Francesca (15)
|
WZAS |
5:08.94 |
5:09.09 |
0.15 |
| 2 |
Smit, Gabrielle M (15)
|
WZAS |
5:10.36 |
5:24.04 |
13.68 |
| 3 |
Yi, Jinyoung (16)
|
WZAS |
5:28.60 |
5:29.02 |
0.42 |
| 4 |
Christianson, Caroline N (16)
|
PVMACH |
5:47.84 |
5:31.41 |
-16.43 |
| 5 |
Webb, Laila R (15)
|
PVMACH |
5:49.84 |
5:51.10 |
1.26 |
| 6 |
Wheeler, AnQuniece C (17)
|
MIDRST |
5:40.79 |
5:51.35 |
10.56 |
| 7 |
Surrey, Mackenzie R (16)
|
WZAS |
6:00.87 |
6:02.68 |
1.81 |
| 8 |
Kaplan, Hanne L (15)
|
PVMACH |
5:59.22 |
6:03.51 |
4.29 |
| 9 |
Kendricks, Kayla L (15)
|
MIDRST |
5:49.70 |
6:04.23 |
14.53 |
| 10 |
Manley, Caitlin E (15)
|
PVVLAC |
6:25.08 |
6:32.06 |
6.98 |
|
Smith, Mellany A (16)
|
NJCAT |
5:32.72 |
NS |
- |
|
Doody, Claire L (15)
|
PVMACH |
6:18.13 |
NS |
- |
|
Lee, Isabella C (17)
|
PVMACH |
5:12.90 |
NS |
- |