| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Varozza, Preston M (20)
|
STUN |
51.90 |
51.78 |
-0.12 |
| 2 |
Lindley, Christohper B (17)
|
NTNTN |
57.10 |
55.43 |
-1.67 |
| 3 |
Godso, Landon T (16)
|
LACRAW |
57.55 |
57.17 |
-0.38 |
| 4 |
Bobbili, Tarun A (16)
|
GUECS |
59.89 |
57.61 |
-2.28 |
| 5 |
Gates, Cooper K (17)
|
STMSC |
58.00 |
58.00 |
0.00 |
| 6 |
Walters, Christian (16)
|
STAAAA |
57.70 |
58.20 |
0.50 |
| 7 |
Swanner, Mason R (17)
|
GUPACK |
58.52 |
58.94 |
0.42 |
| 8 |
Rodriguez, Morgan R (13)
|
STNTRO |
1:02.40 |
1:00.91 |
-1.49 |
| 9 |
Mathews, Georgianna E (15)
|
GURICE |
1:01.68 |
1:01.15 |
-0.53 |
| 10 |
Dodson, Ainsley E (15)
|
GURICE |
1:01.68 |
1:01.49 |
-0.19 |
| 11 |
Evans, Amy L (15)
|
GUDADS |
1:03.80 |
1:04.36 |
0.56 |
| 12 |
Gianotti, Emily J (14)
|
STAAAA |
1:03.68 |
1:04.60 |
0.92 |