| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Hunter C (17)
|
STLCA |
59.55 |
59.22 |
-0.33 |
| 2 |
Prior, Luke M (14)
|
STAAAA |
1:04.46 |
1:00.72 |
-3.74 |
| 3 |
Kelber, Mason A (17)
|
NTNTN |
1:05.25 |
1:01.90 |
-3.35 |
| 4 |
Walters, Christian (16)
|
STAAAA |
1:02.50 |
1:01.97 |
-0.53 |
| 5 |
Bobbili, Tarun A (16)
|
GUECS |
1:06.71 |
1:02.68 |
-4.03 |
| 6 |
Willetts, Benjamin C (15)
|
GUPACK |
1:07.80 |
1:05.58 |
-2.22 |
| 7 |
Gianotti, Emily J (14)
|
STAAAA |
1:05.83 |
1:05.77 |
-0.06 |
| 8 |
Daniels, Richelle P (21)
|
NTFAST |
1:10.88 |
1:09.40 |
-1.48 |
| 9 |
Dodson, Ainsley E (15)
|
GURICE |
1:13.19 |
1:09.83 |
-3.36 |
| 10 |
Lynch, Zoe B (14)
|
STTXLA |
1:16.19 |
1:10.96 |
-5.23 |
| 11 |
Beddingfield, Brooke A (18)
|
GUPACK |
1:11.46 |
1:11.59 |
0.13 |
| 12 |
Schlegel, Hanna H (15)
|
GUSSS |
1:11.50 |
1:14.66 |
3.16 |
|
Perry, Isabella T (17)
|
NTLAC |
1:11.69 |
DQ |
- |