| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bulseco, Jacob E (16)
|
GUSSS |
21.99 |
22.35 |
0.36 |
| 2 |
Moser, Ashton J (16)
|
GULIFE |
22.37 |
22.93 |
0.56 |
| 3 |
Reno, Seth D (15)
|
GUSSS |
23.56 |
22.94 |
-0.62 |
| 4 |
Conroy, Skyler T (16)
|
GUSTA |
22.38 |
23.19 |
0.81 |
| 5 |
MacLeod-Boyd, Alexander C (16)
|
GUBLST |
24.54 |
23.47 |
-1.07 |
| 6 |
Armstrong, David J (15)
|
GUUN-L |
23.12 |
24.10 |
0.98 |
| 7 |
Barrera, Martin I (16)
|
GUNOCH |
23.53 |
24.48 |
0.95 |
| 8 |
Armstrong, Jonathan W (43)
|
GUUN-L |
23.97 |
24.50 |
0.53 |
| 9 |
Gallagher, Connor G (15)
|
GULIFE |
23.17 |
24.65 |
1.48 |
| 10 |
Duport, Shae Q (15)
|
GUSSS |
25.43 |
25.19 |
-0.24 |
| 11 |
Twomey, Zachary A (15)
|
GUSTA |
24.41 |
25.65 |
1.24 |
| 12 |
Null, Cade T (16)
|
GUBLST |
25.55 |
25.75 |
0.20 |
| 13 |
Null, Hunter R (15)
|
GUBLST |
27.43 |
26.78 |
-0.65 |
| 14 |
Gonzalez, Isaac (15)
|
GUNOCH |
26.88 |
26.80 |
-0.08 |
| 15 |
Zepeda, Aaron (15)
|
GUAMBU |
|
30.83 |
- |
| 16 |
McDaniel, Austin J (16)
|
GULIFE |
|
34.12 |
- |
|
Berry, Johnathon M (15)
|
GUAMBU |
28.00 |
NS |
- |
|
Stanley, Jordan C (15)
|
GUSSS |
|
NS |
- |
|
Yanez, Alejandro W (18)
|
GUSSS |
22.67 |
NS |
- |
|
Rosacia, Luther (16)
|
GUNOCH |
56.23 |
NS |
- |
|
Wood, Brock C (15)
|
GUSTA |
26.39 |
NS |
- |