| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lopez, Guadalupe (8)
|
GUBLST |
26.61 |
24.50 |
-2.11 |
| 2 |
Ma, Joanna J (8)
|
GUSSS |
26.22 |
25.56 |
-0.66 |
| 3 |
Prior, Charlotte G (8)
|
GUSSS |
27.10 |
25.68 |
-1.42 |
| 4 |
McEvoy, Charlee F (8)
|
GUSWAT |
|
25.72 |
- |
| 5 |
McGonagle, Brielle (8)
|
GUSSS |
27.39 |
26.11 |
-1.28 |
| 5 |
Dickamore, Kathrin I (8)
|
GUSHRK |
26.29 |
26.11 |
-0.18 |
| 7 |
Montgomery, Emerson E (8)
|
GULJAC |
|
26.72 |
- |
| 8 |
Willemse, Tara A (8)
|
GUCOOG |
31.47 |
27.71 |
-3.76 |
| 9 |
Sisung, Lilly L (8)
|
GUSHRK |
29.25 |
27.88 |
-1.37 |
| 10 |
Kitu, Debra J (8)
|
GUSCAT |
|
28.70 |
- |
| 11 |
Sinha, Anisa J (8)
|
GUCOOG |
|
28.75 |
- |
| 12 |
Dawlett, Natalie M (8)
|
GUSSS |
|
28.89 |
- |
| 13 |
Allis, Sophia F (8)
|
GUSHRK |
38.35 |
34.61 |
-3.74 |
| 14 |
Le, Lyna D (9)
|
GUSHRK |
|
37.25 |
- |
| 15 |
Young, Vivian A (8)
|
GUSSS |
|
38.30 |
- |
| 16 |
Kato, Shiki (7)
|
GUAQUA |
|
38.75 |
- |
|
Moore, Kivlyn A (5)
|
GUSHRK |
37.20 |
DQ |
- |
|
Jacobson, Katherine R (8)
|
GUSHRK |
|
DQ |
- |
|
Battle, Kara M (8)
|
GUSSS |
|
DQ |
- |
|
Ekhaese, Omosede (7)
|
GUSSS |
|
DQ |
- |
|
Bhakta, Aniya K (8)
|
GUSHRK |
|
DQ |
- |
|
Gray, Stormie (8)
|
GUSHRK |
|
NS |
- |