| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scruggs, Emma S (16)
|
SESES |
2:21.80 |
2:23.60 |
1.80 |
| 2 |
Payne, Grayson E (16)
|
SESES |
2:21.25 |
2:23.99 |
2.74 |
| 3 |
Osburn, Aspyn N (17)
|
WTWT |
2:28.41 |
2:25.65 |
-2.76 |
| 4 |
Knorr, Emily S (15)
|
NCNCS |
2:26.23 |
2:25.96 |
-0.27 |
| 5 |
Summers, Anna L (15)
|
GAGA |
2:27.10 |
2:26.04 |
-1.06 |
| 6 |
Smith, Olivia R (17)
|
SESES |
2:23.44 |
2:28.22 |
4.78 |
| 7 |
Brown, Sarah E (15)
|
GAGA |
2:25.24 |
2:29.15 |
3.91 |
| 8 |
Bertschi, Samantha M (15)
|
GAGA |
2:29.92 |
2:29.50 |
-0.42 |
| 9 |
Knorr, Kathryn A (15)
|
NCNCS |
2:27.12 |
2:29.95 |
2.83 |
| 10 |
Stallworth, Aspen A (15)
|
STSTX |
2:27.26 |
2:30.04 |
2.78 |
| 11 |
Whitaker, Peyton A (17)
|
NCNCS |
2:31.95 |
2:31.90 |
-0.05 |
| 12 |
Flynn, Reagan A (15)
|
FLFL |
2:32.18 |
2:32.00 |
-0.18 |
| 13 |
Linkous, Reagan M (16)
|
WVWV |
2:36.36 |
2:32.08 |
-4.28 |
| 14 |
Wong, Katie C (15)
|
GAGA |
2:30.22 |
2:32.31 |
2.09 |
| 15 |
Berger, Julie C (17)
|
SESES |
2:26.13 |
2:33.47 |
7.34 |
| 16 |
Harper, Kathleen C (16)
|
GAGA |
2:25.48 |
2:35.09 |
9.61 |
| 17 |
Wissinger, Emily M (16)
|
FLFL |
2:32.64 |
2:36.36 |
3.72 |
| 18 |
Gamez, Bella M (15)
|
STSTX |
2:30.88 |
2:37.60 |
6.72 |
| 19 |
Foreman, Addy A (15)
|
WTWT |
2:39.66 |
2:39.14 |
-0.52 |
| 20 |
Engel, Jessica E (15)
|
STSTX |
2:31.57 |
2:40.58 |
9.01 |
| 21 |
Layne, MacKenzie G (15)
|
WVWV |
2:35.64 |
2:41.85 |
6.21 |
| 22 |
Muller, Taylor E (15)
|
BDBD |
2:42.32 |
2:41.97 |
-0.35 |
| 23 |
Rucker, Victoria H (16)
|
GAGA |
2:34.82 |
2:42.94 |
8.12 |
| 24 |
Vanginault, Christine (17)
|
WTWT |
2:53.20 |
2:48.77 |
-4.43 |
| 25 |
Greenlee, Madelyn A (16)
|
KYKY |
2:40.42 |
2:50.32 |
9.90 |
| 26 |
Fenton, Ember (16)
|
WTWT |
3:12.01 |
3:00.60 |
-11.41 |