| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Langley, Stephen L (15)
|
GUBTA |
31.16 |
24.90 |
-6.26 |
| 2 |
Mastracchio, Dominic R (44)
|
GUUN-T |
25.00 |
24.94 |
-0.06 |
| 3 |
Doehring, Caleb J (17)
|
GUBTA |
25.76 |
25.04 |
-0.72 |
| 4 |
Pickering, Owen R (16)
|
GUBTA |
32.19 |
25.16 |
-7.03 |
| 5 |
Hunt, Braden T (16)
|
GUBTA |
39.18 |
25.57 |
-13.61 |
| 6 |
Smith, Daniel A (15)
|
GUESA |
25.70 |
26.03 |
0.33 |
| 7 |
Ozenne, Conner J (17)
|
GUESA |
25.29 |
27.07 |
1.78 |
| 8 |
Conway, William J (16)
|
GUBTA |
26.34 |
27.10 |
0.76 |
| 9 |
Rosenwinkel, William C (18)
|
GUBTA |
27.18 |
27.18 |
0.00 |
| 10 |
Agmata, Ian C (15)
|
GUCATS |
27.78 |
27.56 |
-0.22 |
| 11 |
Sze, Matthew H (16)
|
GUSPA |
35.79 |
29.82 |
-5.97 |
| 12 |
Stevens, Jaxon J (15)
|
GUBTA |
|
30.12 |
- |
| 13 |
Cox, Andrew L (15)
|
GUESA |
|
30.50 |
- |
| 14 |
Montgomery, Luke Z (15)
|
GUOFSC |
|
32.31 |
- |
|
Chen, Edward Y (16)
|
GUSPA |
27.05 |
NS |
- |