| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simmons, Lucy B (5)
|
GUESA |
34.32 |
25.94 |
-8.38 |
| 2 |
Alcala, Cecile N (6)
|
GUBTA |
28.11 |
26.02 |
-2.09 |
| 3 |
Caver, Laila O (6)
|
GUSPA |
30.63 |
26.10 |
-4.53 |
| 4 |
Weikel, Trinity G (6)
|
GUESA |
25.36 |
26.45 |
1.09 |
| 5 |
Burns, Amelia M (6)
|
GUTWST |
26.21 |
26.63 |
0.42 |
| 6 |
Castaneda, Viviana I (6)
|
GUTWST |
40.99 |
28.14 |
-12.85 |
| 7 |
Messick, Avery M (5)
|
GUTWST |
|
28.32 |
- |
| 8 |
Bayer, Marie K (6)
|
GUTWST |
30.71 |
28.92 |
-1.79 |
| 9 |
Bodnar, Ava L (5)
|
GUTWST |
45.99 |
30.40 |
-15.59 |
| 10 |
Yakopich, Reese V (6)
|
GUSPA |
38.92 |
30.75 |
-8.17 |
| 11 |
Lindberg, Lucy A (5)
|
GUBTA |
32.27 |
31.16 |
-1.11 |
| 12 |
Rojas, Allison J (5)
|
GUTWST |
39.72 |
31.26 |
-8.46 |
| 13 |
Trivedi, Srishti B (6)
|
GUSPA |
|
32.71 |
- |
| 14 |
Graves, Willa M (6)
|
GUTWST |
29.25 |
33.43 |
4.18 |
| 15 |
Castor, Jordyn A (5)
|
GUTWST |
50.99 |
33.91 |
-17.08 |
| 16 |
Caver, Lauren A (5)
|
GUSPA |
|
35.48 |
- |
| 17 |
Espinosa-Agrait, Juliana I (5)
|
GUTWST |
|
36.45 |
- |
| 18 |
Amezcua, Sarah E (6)
|
GUSWIM |
42.00 |
41.42 |
-0.58 |
| 19 |
McEwen, Raegan L (5)
|
GUOFSC |
|
51.57 |
- |
| 20 |
Villarreal, Lillianna H (4)
|
GUBTA |
55.09 |
1:00.74 |
5.65 |
| 21 |
Guynes, Emerson N (5)
|
GUESA |
|
1:03.60 |
- |
|
Robertson, Sutton C (5)
|
GUESA |
|
NS |
- |