| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burke, Anna E (12)
|
GUPEAK |
5:58.09 |
5:57.08 |
-1.01 |
| 2 |
Bleyendaal, Rian C (12)
|
GUPEAK |
|
5:57.36 |
- |
| 3 |
Stibrich, Lizzy A (12)
|
GUBTA |
5:58.95 |
6:00.73 |
1.78 |
| 4 |
Fernandes, Helena W (12)
|
GUPEAK |
5:48.62 |
6:02.99 |
14.37 |
| 5 |
Bremer, Lorelai A (11)
|
GUPEAK |
6:04.38 |
6:03.13 |
-1.25 |
| 6 |
Stowers, Sophie M (12)
|
GUPEAK |
6:21.71 |
6:11.79 |
-9.92 |
| 7 |
James, Audrey M (12)
|
GUBTA |
|
6:17.85 |
- |
| 8 |
Chinen, Marisa A (11)
|
GUBTA |
6:54.30 |
6:28.35 |
-25.95 |
| 9 |
Molina, Leah K (12)
|
GUBTA |
6:48.71 |
6:28.84 |
-19.87 |
| 10 |
Thibodeaux, Samantha B (12)
|
GUBTA |
|
6:44.79 |
- |
| 11 |
Scott, Courtney L (11)
|
GUBTA |
|
6:46.17 |
- |
| 12 |
Donahue, Christina M (11)
|
GUBTA |
7:01.44 |
6:52.84 |
-8.60 |
| 13 |
Richey, Madeline K (11)
|
GUAMBU |
7:01.53 |
7:10.42 |
8.89 |
| 14 |
Vose, Haylee E (12)
|
GUAMBU |
7:13.74 |
7:23.93 |
10.19 |
| 15 |
Johnston, Ashlynne D (12)
|
GUBTA |
|
7:31.78 |
- |
| 16 |
Luttrell, Hailey C (11)
|
GUBTA |
|
7:55.19 |
- |