| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murray, Ryan G (12)
|
GUBTA |
34.03 |
33.44 |
-0.59 |
| 2 |
McFarlain, Gavin R (12)
|
GUNEHA |
34.82 |
34.85 |
0.03 |
| 3 |
Trent, Ryder R (12)
|
GUBTA |
34.59 |
35.04 |
0.45 |
| 4 |
James, Trevor (11)
|
GUBTA |
36.63 |
35.09 |
-1.54 |
| 5 |
Zbylot, Stanton E (11)
|
GUAMBU |
50.47 |
39.94 |
-10.53 |
| 6 |
Flosser, Nathaniel M (11)
|
GUSPA |
39.33 |
40.54 |
1.21 |
| 7 |
Baker Graham, Ezrah (12)
|
GUNEHA |
|
43.84 |
- |
| 8 |
Zheng, Jeffrey (11)
|
GUAMBU |
|
50.49 |
- |
| 9 |
McElroy, Evan G (12)
|
GUAMBU |
|
52.99 |
- |
| 10 |
Farish, Matias I (11)
|
GUPLAT |
51.82 |
53.56 |
1.74 |
| 11 |
Kuzminski, Austin B (11)
|
GUBTA |
|
57.19 |
- |
| 12 |
Smith, Landon P (11)
|
GUAMBU |
55.73 |
57.65 |
1.92 |
|
Verano, Vincent Jon A (11)
|
GUSPA |
51.76 |
NS |
- |
|
Sola, Giann L (12)
|
GUSPA |
43.92 |
NS |
- |
|
Quick, Charlie (11)
|
GUSPA |
39.53 |
NS |
- |
|
McKinney, Braxton F (12)
|
GUSPA |
36.05 |
NS |
- |
|
Reitmeyer, William F (11)
|
GUNEHA |
43.84 |
NS |
- |
|
Rodriguez, Bryan N (11)
|
GUAMBU |
|
NS |
- |