| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhao, Johnathan M (10)
|
GUPACK |
48.14 |
45.28 |
-2.86 |
| 2 |
Ortiz, Noah J (10)
|
GUPEAK |
48.06 |
46.77 |
-1.29 |
| 3 |
Nguyen, Tom T (10)
|
GUESCA |
52.05 |
47.15 |
-4.90 |
| 4 |
Nguyen, Harrison K (8)
|
GUESCA |
53.19 |
50.37 |
-2.82 |
| 5 |
Wright, Matthew S (10)
|
GUPACK |
58.65 |
50.57 |
-8.08 |
| 6 |
Keck, Richard J (9)
|
GUPEAK |
52.72 |
50.86 |
-1.86 |
| 7 |
Doan, Dreyden C (9)
|
GULSST |
44.34 |
52.26 |
7.92 |
| 8 |
Galvez, Vincent R (9)
|
GUESCA |
46.54 |
53.03 |
6.49 |
| 9 |
Silva, Diego A (10)
|
GUESCA |
47.89 |
53.21 |
5.32 |
| 10 |
Gu, Allen * (9)
|
GUPEAK |
|
53.33 |
- |
| 11 |
Davila, Santiago (10)
|
GUPACK |
55.55 |
53.76 |
-1.79 |
| 12 |
Weaver, Jacob E (10)
|
GUPEAK |
58.53 |
54.57 |
-3.96 |
| 13 |
Shah, Jonathan K (9)
|
GUPACK |
57.22 |
54.96 |
-2.26 |
| 14 |
Samuels, Ethan L (10)
|
GUPACK |
|
55.43 |
- |
| 15 |
Saraya, Ibrahim Jr M (10)
|
GUESCA |
59.04 |
55.46 |
-3.58 |
| 16 |
Lanigan, Daniel B (10)
|
GUAMBU |
|
55.63 |
- |
| 17 |
Watkins, Michael E (10)
|
GUPEAK |
|
55.89 |
- |
| 18 |
Camarena, Alejandro * (9)
|
GUPEAK |
1:01.95 |
57.17 |
-4.78 |
| 19 |
Canovas, William W (10)
|
GUPACK |
|
57.37 |
- |
| 20 |
Roberts, Karim M (10)
|
GUPACK |
1:05.75 |
57.57 |
-8.18 |
| 21 |
Wesley, Caleb M (10)
|
GUPEAK |
1:04.02 |
57.64 |
-6.38 |
| 22 |
Kim, Peter S (10)
|
GUKATY |
50.18 |
58.95 |
8.77 |
| 23 |
Wiesenborn, Carson S (10)
|
GUKATY |
1:00.39 |
59.18 |
-1.21 |
| 24 |
Castellanos, Hunter J (10)
|
GUKATY |
|
1:04.11 |
- |
| 25 |
Wildermuth, Benjamin R (10)
|
GUPACK |
|
1:04.98 |
- |
| 26 |
Roberts, Omar S (10)
|
GUPACK |
|
1:08.48 |
- |
|
Sullivan, Seth R (10)
|
GUAMBU |
|
DQ |
- |