| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jayr, Amaury B (14)
|
GUDADS |
30.37 |
25.18 |
-5.19 |
| 2 |
Nace, Jackson E (14)
|
GUTYES |
31.71 |
25.30 |
-6.41 |
| 3 |
Chin, Thomas Zhee Yang (14)
|
GUECS |
33.21 |
25.66 |
-7.55 |
| 4 |
Boyes, Ethan E (14)
|
GUAGS |
28.30 |
25.76 |
-2.54 |
| 5 |
Sancho-Spore, Alexander D (14)
|
GUCFSC |
28.17 |
25.87 |
-2.30 |
| 6 |
Itkins, Daniel R (14)
|
GUECS |
27.08 |
25.96 |
-1.12 |
| 7 |
Choi, Lennox (14)
|
GUECS |
25.89 |
25.99 |
0.10 |
| 8 |
Lindberg, Calvin B (14)
|
GUAGS |
32.85 |
26.49 |
-6.36 |
| 9 |
Brooks, Joshua C (13)
|
GUMAC |
30.41 |
26.59 |
-3.82 |
| 9 |
Daugard, Caden A (13)
|
GUBATS |
27.77 |
26.59 |
-1.18 |
| 11 |
Borak, Brock D (14)
|
GUVSST |
30.68 |
26.65 |
-4.03 |
| 12 |
Pedro, Ethnan F (14)
|
GUDADS |
31.39 |
26.76 |
-4.63 |
| 12 |
Jimenez, Isaac N (13)
|
GUTEST |
35.44 |
26.76 |
-8.68 |
| 14 |
Ridley, William J (13)
|
GUSSAN |
26.74 |
26.91 |
0.17 |
| 15 |
Taylor, Christian A (14)
|
GUTASC |
34.69 |
27.05 |
-7.64 |
| 16 |
Huye, Josh S (14)
|
GUSPA |
33.18 |
27.07 |
-6.11 |
| 17 |
Belmar, Quinn D (13)
|
GUDADS |
29.69 |
27.30 |
-2.39 |
| 18 |
Sorensen, Charles J (14)
|
GUMAC |
29.94 |
27.31 |
-2.63 |
| 19 |
Putnam, Owen D (14)
|
GUCFSC |
28.83 |
27.61 |
-1.22 |
| 20 |
Saria, Nathan D (13)
|
GUTYES |
26.99 |
27.69 |
0.70 |
| 21 |
McDonald, Christopher R (14)
|
GUUN-B |
32.75 |
27.72 |
-5.03 |
| 22 |
Putnam, Jack E (14)
|
GUCFSC |
26.79 |
27.96 |
1.17 |
| 23 |
Walker, William Jack (14)
|
GUECS |
28.06 |
28.10 |
0.04 |
| 24 |
Mi, Brayden K (13)
|
GUECS |
|
28.25 |
- |
| 25 |
Angulo, Gerardo (14)
|
GUPFL |
34.23 |
28.78 |
-5.45 |
| 26 |
Johnson, Carston (13)
|
GUAGS |
33.80 |
29.14 |
-4.66 |
| 27 |
Snyder, Jayson D (13)
|
GUTASC |
35.09 |
30.04 |
-5.05 |
|
Woodall, William G (14)
|
GUSST |
35.72 |
NS |
- |
|
DeLeon, Macario B (13)
|
GUAGS |
28.15 |
NS |
- |