| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Itkins, Daniel R (14)
|
GUECS |
26.78 |
27.22 |
0.44 |
| 2 |
Ridley, William J (13)
|
GUSSAN |
27.07 |
27.48 |
0.41 |
| 3 |
Borak, Brock D (14)
|
GUVSST |
29.29 |
27.76 |
-1.53 |
| 4 |
Dharbal, Alexander N (14)
|
GUSWIM |
28.18 |
27.77 |
-0.41 |
| 5 |
Sorensen, Charles J (14)
|
GUMAC |
|
27.78 |
- |
| 6 |
Daugard, Caden A (13)
|
GUBATS |
29.27 |
28.10 |
-1.17 |
| 7 |
Choi, Lennox (14)
|
GUECS |
29.66 |
28.51 |
-1.15 |
| 8 |
Taylor, Christian A (14)
|
GUTASC |
30.34 |
28.56 |
-1.78 |
| 9 |
Putnam, Jack E (14)
|
GUCFSC |
32.80 |
29.02 |
-3.78 |
| 10 |
Walker, William Jack (14)
|
GUECS |
31.13 |
29.74 |
-1.39 |
| 11 |
Jimenez, Isaac N (13)
|
GUTEST |
39.30 |
30.18 |
-9.12 |
| 12 |
Mi, Brayden K (13)
|
GUECS |
|
31.09 |
- |
|
DeLeon, Macario B (13)
|
GUAGS |
32.19 |
NS |
- |
|
Rice, Levi E (14)
|
GUAGS |
|
NS |
- |
|
Woodall, William G (14)
|
GUSST |
30.88 |
NS |
- |