| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huang, Angelina (12)
|
NTDM |
2:23.18 |
2:21.10 |
-2.08 |
| 2 |
Witte, Carissa R (12)
|
NTLTSD |
2:42.22 |
2:29.51 |
-12.71 |
| 3 |
Acuff, Sienna B (11)
|
NTDM |
2:30.04 |
2:31.74 |
1.70 |
| 4 |
Jacobson, Cara J (12)
|
NTDM |
2:32.22 |
2:32.17 |
-0.05 |
| 5 |
Rickard, Mikaela-Mae K (11)
|
NTATAC |
2:34.49 |
2:33.72 |
-0.77 |
| 6 |
Estrada, Natalie (11)
|
NTDM |
2:34.59 |
2:33.96 |
-0.63 |
| 7 |
Chang, Rosalind H (12)
|
NTDM |
2:32.89 |
2:34.29 |
1.40 |
| 8 |
Cagle, Brynnlee R (11)
|
NTMTRO |
2:52.93 |
2:41.18 |
-11.75 |
| 9 |
Gyure, Katherine G (12)
|
NTMTRO |
2:54.35 |
2:45.87 |
-8.48 |
| 10 |
VanVossen, Morgan M (11)
|
GUMARC |
3:10.47 |
2:48.44 |
-22.03 |
| 11 |
Smith, Katelyn N (12)
|
GUMARC |
2:54.97 |
2:49.29 |
-5.68 |
| 12 |
Fox, Callan M (11)
|
NTESP |
2:49.96 |
2:49.61 |
-0.35 |
| 13 |
Evans, Samantha C (11)
|
NTMTRO |
3:08.33 |
2:52.44 |
-15.89 |
| 14 |
Sellers, Rachel J (12)
|
NTLTSD |
3:11.98 |
2:53.92 |
-18.06 |
| 15 |
Steger, Elizabeth A (12)
|
NTDM |
3:01.59 |
2:54.09 |
-7.50 |
| 16 |
Pearson, Tori S (11)
|
NTMTRO |
|
2:54.12 |
- |
| 17 |
Callan, Emery M (12)
|
NTCOPS |
3:06.73 |
2:54.31 |
-12.42 |
| 18 |
Krupinski, Haley R (12)
|
NTCOPS |
2:59.42 |
2:57.72 |
-1.70 |
| 19 |
Ellis, Caroline G (12)
|
NTESP |
2:56.82 |
2:57.81 |
0.99 |
| 20 |
Payne, Addison R (12)
|
GUMARC |
3:04.56 |
3:02.11 |
-2.45 |
| 21 |
Brook, Elyana A (11)
|
NTDM |
3:08.07 |
3:03.22 |
-4.85 |
| 22 |
Bailey, Ava A (11)
|
NTJMSS |
3:03.56 |
3:03.55 |
-0.01 |
| 23 |
Norris, Audrey K (12)
|
NTJMSS |
3:22.94 |
3:05.66 |
-17.28 |
| 24 |
Graham, Riley G (11)
|
NTCOPS |
3:16.71 |
3:05.85 |
-10.86 |
| 25 |
Breuer, Callie E (12)
|
NTCOPS |
3:06.97 |
3:06.19 |
-0.78 |
| 26 |
Nelson, Lundynn E (11)
|
NTUN |
3:19.65 |
3:11.23 |
-8.42 |
| 27 |
Ronsman, Lauren E (11)
|
GUMARC |
3:25.91 |
3:18.28 |
-7.63 |
| 28 |
Kohok, Aneesha (11)
|
NTCOPS |
|
3:19.58 |
- |
| 29 |
Crissman, Isabella R (11)
|
NTJMSS |
3:31.39 |
3:22.09 |
-9.30 |
| 30 |
Nelson, Kate M (11)
|
NTCOPS |
|
3:27.08 |
- |