| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodgers, Nora Jane M (12)
|
GUFRST |
2:51.51 |
2:32.95 |
-18.56 |
| 2 |
Zhang, Amy * (11)
|
GUPEAK |
3:12.06 |
2:39.49 |
-32.57 |
| 3 |
Duncan, Grace K (12)
|
GUESA |
|
2:40.51 |
- |
| 4 |
Orsi, Emma E (11)
|
GUPEAK |
2:47.85 |
2:41.33 |
-6.52 |
| 5 |
Koncaba, Alice J (12)
|
GUSHRK |
2:56.47 |
2:45.33 |
-11.14 |
| 6 |
Pineda, Maria J (12)
|
GUESA |
2:52.88 |
2:50.08 |
-2.80 |
| 7 |
Smith, Caylin I (11)
|
GUPLAT |
3:24.49 |
2:52.87 |
-31.62 |
| 8 |
DeGuise, Emerson P (11)
|
GUTYES |
|
2:54.17 |
- |
| 9 |
Neilon, Sydney J (12)
|
GUPEAK |
3:10.39 |
2:56.47 |
-13.92 |
| 10 |
Esbeck, Olivia J (11)
|
GUSHRK |
2:54.97 |
3:01.07 |
6.10 |
| 11 |
Ferrero D'Ambrosio, Kira (12)
|
GUTYES |
|
3:01.47 |
- |
| 12 |
Boettcher, Kyleigh M (11)
|
GUSWAT |
3:02.34 |
3:02.08 |
-0.26 |
|
Brown, Allison A (12)
|
GUPEAK |
|
DQ |
- |