| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nikolakopoulos, Sofia A (11)
|
GUESCA |
2:40.52 |
2:43.09 |
2.57 |
| 2 |
Hernandez, Sofia Z (12)
|
GUCFSC |
2:49.95 |
2:48.56 |
-1.39 |
| 3 |
Nguyen, Amelie T (12)
|
GUAQUA |
2:42.27 |
2:48.63 |
6.36 |
| 4 |
Medina, Daniela M (12)
|
GUCFSC |
|
2:50.57 |
- |
| 5 |
Karim, Isabella (12)
|
GUFCST |
2:56.72 |
2:55.48 |
-1.24 |
| 6 |
Tremaine, Malea T (12)
|
GUESCA |
2:44.06 |
2:56.83 |
12.77 |
| 7 |
Norian, Olive F (12)
|
GUFCST |
2:55.56 |
2:57.16 |
1.60 |
| 8 |
Watson, Jimena (11)
|
GUCFSC |
2:50.51 |
2:57.68 |
7.17 |
| 9 |
Quirsfeld, Eryn L (12)
|
GUCFSC |
|
3:00.53 |
- |
| 10 |
Pham, Abigail T (12)
|
GUCFSC |
3:02.46 |
3:00.79 |
-1.67 |
| 11 |
Leggiero, Vittoria V (11)
|
GUSSAN |
|
3:02.66 |
- |
| 12 |
Ly, Yvonne K (11)
|
GUESCA |
2:56.00 |
3:02.71 |
6.71 |
| 13 |
Maddan, Erin F (11)
|
GUCFSC |
|
3:03.79 |
- |
| 14 |
Light, Ava S (11)
|
GUFCST |
3:06.92 |
3:05.50 |
-1.42 |
| 15 |
Tran, Emily H (11)
|
GUESCA |
3:10.92 |
3:06.37 |
-4.55 |
| 16 |
Wolfley, Alexis M (12)
|
GUFCST |
|
3:08.83 |
- |
| 17 |
Shepherd, Gabriella F (11)
|
GUCFSC |
3:21.67 |
3:10.16 |
-11.51 |
| 18 |
Ford, Emily G (12)
|
GUFCST |
3:18.58 |
3:12.69 |
-5.89 |
| 19 |
Shanor, Kalie A (11)
|
GUSSAN |
3:08.21 |
3:15.44 |
7.23 |
| 20 |
Gonzalez, Gabriella R (12)
|
GUCFSC |
3:13.01 |
3:15.64 |
2.63 |
| 21 |
Jaco, Riley N (11)
|
GUSSAN |
3:14.84 |
3:16.53 |
1.69 |
| 22 |
Raval, Niva M (12)
|
GUFCST |
3:14.04 |
3:18.25 |
4.21 |
| 23 |
Baker, Brooke C (12)
|
GUESCA |
3:20.37 |
3:18.41 |
-1.96 |
| 24 |
Fuller, Lauren V (12)
|
GUFCST |
|
3:24.64 |
- |
| 25 |
Pawlak, Ashley B (12)
|
GUSSAN |
3:31.51 |
3:34.76 |
3.25 |
| 26 |
Henderson, Elizabeth C (11)
|
GUSSAN |
|
4:00.66 |
- |