| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nelson, Abigail Y (15)
|
GUSHRK |
59.09 |
59.34 |
0.25 |
| 2 |
Szklaruk-Traipe, Sarah B (17)
|
GUSHRK |
58.19 |
1:01.42 |
3.23 |
| 3 |
Yeguez, Erika B (16)
|
GUSHRK |
1:04.25 |
1:03.33 |
-0.92 |
| 4 |
Moore, Maven A (15)
|
GUSHRK |
1:03.80 |
1:04.45 |
0.65 |
| 5 |
Friedman, Anjali R (16)
|
GUSHRK |
1:02.53 |
1:04.59 |
2.06 |
| 6 |
Waters, Jordyn N (17)
|
GUSHRK |
1:04.52 |
1:05.26 |
0.74 |
| 7 |
Dawlett, Niela T (15)
|
GUSSS |
1:05.95 |
1:06.93 |
0.98 |
| 8 |
Fitzharris, Lauren S (15)
|
GUSHRK |
1:06.56 |
1:11.71 |
5.15 |
| 9 |
Hillegeist, Katelin D (16)
|
GUSHRK |
1:06.94 |
1:12.22 |
5.28 |
| 10 |
Rudberg, Madison B (15)
|
GUTEST |
1:22.40 |
1:16.41 |
-5.99 |
| 11 |
Stewart, Grace I (16)
|
GUTEST |
1:21.84 |
1:17.38 |
-4.46 |
| 12 |
Little, Emily E (16)
|
GUSHRK |
1:29.36 |
1:28.20 |
-1.16 |