| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Luong, Kate Y (10)
|
NTMTRO |
5:43.66 |
5:39.13 |
-4.53 |
| 2 |
Acuff, Sienna B (10)
|
NTDM |
5:47.36 |
5:39.20 |
-8.16 |
| 3 |
Yin, Julia (10)
|
NTCOPS |
5:51.19 |
5:42.82 |
-8.37 |
| 4 |
Schneider, Natalie M (10)
|
NTLAC |
5:51.46 |
5:42.88 |
-8.58 |
| 5 |
Pellegrin, Marley J (10)
|
NTTFA |
6:04.51 |
5:49.18 |
-15.33 |
| 6 |
Farlow, Morgan M (10)
|
NTTFA |
6:10.96 |
5:52.57 |
-18.39 |
| 7 |
Stapleton, Sloane K (10)
|
GUCFSC |
5:54.18 |
5:53.52 |
-0.66 |
| 8 |
McGlohn, Lauren E (10)
|
GUCFSC |
6:12.41 |
5:54.13 |
-18.28 |
| 9 |
Ray, Brylie A (10)
|
NTRACE |
5:58.39 |
5:55.21 |
-3.18 |
| 10 |
Peterson, Penelope L (10)
|
NTLAC |
6:11.64 |
5:56.20 |
-15.44 |
| 11 |
Hosey, Daya C (10)
|
GUPACK |
6:01.93 |
5:56.44 |
-5.49 |
| 12 |
Hu, Grace L (10)
|
NTTFA |
5:58.72 |
5:59.05 |
0.33 |
| 13 |
Underwood, Gabriella J (10)
|
GUSSAN |
6:02.16 |
6:01.74 |
-0.42 |
| 14 |
Corbin, Mackenzie G (9)
|
NTTFA |
6:05.17 |
6:03.39 |
-1.78 |
| 15 |
Chandra, Carissa A (10)
|
NTNTN |
6:04.95 |
6:03.50 |
-1.45 |
| 16 |
Pena, Olivia L (10)
|
STNTRO |
6:07.30 |
6:03.62 |
-3.68 |
| 17 |
Defranco, Sabrina P (10)
|
STNTRO |
6:09.54 |
6:05.57 |
-3.97 |
| 18 |
Guglielmello, Anelle C (9)
|
GUMAC |
6:12.22 |
6:06.50 |
-5.72 |
| 19 |
Bremer, Lorelai A (10)
|
GUPEAK |
6:04.38 |
6:07.61 |
3.23 |
| 20 |
Jones, Kinley A (10)
|
STSASA |
5:59.76 |
6:08.28 |
8.52 |
| 21 |
Estrada, Natalie (10)
|
NTDM |
6:10.79 |
6:09.52 |
-1.27 |
| 22 |
Poole, Brooklyn M (9)
|
GUAGS |
6:12.79 |
6:12.83 |
0.04 |
| 23 |
Crudo, Sofia I (10)
|
GUSSS |
6:08.53 |
6:14.05 |
5.52 |
| 24 |
Wiles, Lily L (10)
|
GUTWST |
6:11.50 |
6:16.15 |
4.65 |