| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williamson, Eloise E (12)
|
GUDADS |
24.77 |
24.69 |
-0.08 |
| 2 |
Shade, Caroline I (12)
|
GUKATY |
25.45 |
25.08 |
-0.37 |
| 3 |
Finley, Haley R (12)
|
GUESCA |
25.02 |
25.10 |
0.08 |
| 4 |
Haupt, Evelyn A (12)
|
NTRACE |
26.09 |
25.11 |
-0.98 |
| 5 |
George, Madison D (12)
|
GUESA |
24.95 |
25.25 |
0.30 |
| 6 |
Anderson, Vivian C (12)
|
NTNTN |
25.21 |
25.27 |
0.06 |
| 6 |
Bruner, Sienna M (12)
|
STLCA |
25.15 |
25.27 |
0.12 |
| 8 |
Luper, Sofia L (12)
|
GUCFSC |
25.69 |
25.59 |
-0.10 |
| 9 |
McQuinn, Ella G (12)
|
STFISH |
26.19 |
25.65 |
-0.54 |
| 10 |
Clem, Marin O (12)
|
NTNTN |
26.05 |
25.69 |
-0.36 |
| 11 |
Cronk, Carli E (12)
|
STAAAA |
25.67 |
25.73 |
0.06 |
| 12 |
Murray, Madison R (12)
|
GUBLST |
26.18 |
25.75 |
-0.43 |
| 13 |
Anderson, Kathleen E (12)
|
GUSSAN |
26.04 |
26.04 |
0.00 |
| 14 |
Mongenel, Ella N (12)
|
STATAC |
25.79 |
26.10 |
0.31 |
| 15 |
Andrews, Kaia J (12)
|
GUPACE |
26.22 |
26.15 |
-0.07 |
| 16 |
Pfannstiel, Sophia E (11)
|
NTCOPS |
26.08 |
26.20 |
0.12 |
| 17 |
Kelner, Calista A (12)
|
STSASA |
26.03 |
26.28 |
0.25 |
| 18 |
Butler, Allegra R (12)
|
GUCFSC |
26.17 |
26.30 |
0.13 |
| 19 |
Villatoro, Lindsay K (11)
|
STAAAA |
26.39 |
26.37 |
-0.02 |
| 20 |
Boettcher, Kadyn S (12)
|
GUSWAT |
26.35 |
26.44 |
0.09 |
| 21 |
Chen, Olivia J (12)
|
NTLAC |
29.64 |
26.46 |
-3.18 |
| 22 |
Hartman, Dez Rae Z (12)
|
STSASA |
26.22 |
26.49 |
0.27 |
| 23 |
Shimel, Julia A (12)
|
GUESA |
26.15 |
26.50 |
0.35 |
| 24 |
Jeanes, Andelynn E (12)
|
GUSHRK |
25.50 |
26.51 |
1.01 |
| 25 |
Mulloy, Raegan L (12)
|
GUPACK |
26.48 |
26.69 |
0.21 |
| 26 |
Prabhakar, Haasini (11)
|
GUCFSC |
26.37 |
26.80 |
0.43 |
| 27 |
Jiampietro, Alexandra J (12)
|
STNTRO |
26.30 |
26.88 |
0.58 |
| 28 |
Shirley, Brooke L (12)
|
NTRACE |
26.29 |
27.03 |
0.74 |
| 29 |
Adair, Sydney E (12)
|
GUESA |
26.25 |
27.13 |
0.88 |