| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mills, Justin T (17)
|
PCPASA |
46.40 |
45.88 |
-0.52 |
| 2 |
Nielsen D'Orsi, Felipe (18)
|
NTLAC |
46.15 |
46.87 |
0.72 |
| 3 |
Navarro, Xavier A (18)
|
BDEPAP |
47.90 |
48.01 |
0.11 |
| 4 |
Macleod-Boyd, Alexander C (17)
|
GUBLST |
50.29 |
48.70 |
-1.59 |
| 4 |
Gallardo, Alejandro (16)
|
BDEPAP |
47.70 |
48.70 |
1.00 |
| 6 |
Bobbili, Tarun A (17)
|
GUECS |
50.50 |
48.96 |
-1.54 |
| 7 |
Louie, Joshua S (16)
|
STWLOO |
50.05 |
49.14 |
-0.91 |
| 8 |
Parry, William E (17)
|
GUFCST |
50.52 |
49.27 |
-1.25 |
| 9 |
Pogue, Jackson W (15)
|
NTNTN |
48.67 |
49.56 |
0.89 |
| 10 |
Godso, Landon T (17)
|
LACRAW |
49.72 |
49.82 |
0.10 |
| 11 |
Castillo-Danforth, Ismael E (16)
|
GUFCST |
50.66 |
49.88 |
-0.78 |
| 12 |
Thomas, Nathaniel W (17)
|
STWLOO |
50.75 |
50.15 |
-0.60 |
| 13 |
Schoenthaler, Robert B (16)
|
PCPASA |
52.20 |
50.43 |
-1.77 |
| 14 |
Culligan, Alexander M (16)
|
GUECS |
51.61 |
50.98 |
-0.63 |